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<p>Plant diseases caused by fungi, bacteria, and viruses pose a critical threat to global food security. Chemical pesticides are ineffective and might be harmful to the environment. Hence, there is an urgent need to develop alternative and eco-friendly strategies for managing plant diseases. Nanobiotechnology, particularly the use of silver nanoparticles (AgNPs), offers a promising solution. Antagonistic bacteria, due to their natural antimicrobial properties, can serve as effective agents for nanoparticle synthesis. This study investigates the green (bacteria-mediated) synthesis of AgNPs using <i>Pseudomonas fluorescens</i> as an eco-friendly approach to control <i>Cochliobolus miyabeanus</i>, the source of brown leaf in rice. The synthesis of AgNPs was facilitated by <i>Pseudomonas fluorescens</i>, utilizing bacterial biomolecules and enzymes to catalyze nanoparticle formation. FTIR analysis revealed the involvement of secondary metabolites, identified the functional group in the 3434–400&#xa0;cm⁻<sup>1</sup> range. The nanoparticles were further characterized using UV–Vis spectroscopy, SEM (revealing spherical, rod, and cubic shapes), and EDX (showing 32.93% silver content), with an average size of the particle is 50&#xa0;nm. Morphological changes, such as hyphal tip swelling, bursting, and structural deformation observed in <i>C. miyabeanus</i> upon exposure to AgNPs, highlight their potent fungicidal activity, demonstrating the efficacy of green-synthesized AgNPs as sustainable biocontrol agents. Thus, <i>Pseudomonas fluorescens</i> emerges as a promising candidate for antifungal activity against brown leaf spot in rice, offering a viable, environmentally friendly alternative to chemical pesticides.</p>

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Pseudomonas fluorescens–Based Biogenic Silver Nanoparticles: a Green Solution for Brown Leaf Spot Disease in Rice

  • 期刊论文

  • 发表日期：

    2025年4月2日

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  • Ebenezar Immanuel J,
  • Muthukumar Balamurugan,
  • Santhosh Ram R Prabhuram,
  • Govindarajan Venkat Kumar,
  • Ashraf Chamseddine,
  • Palaniyandi Velusamy,
  • Majed A. Bajaber,
  • Subash C. B. Gopinath

摘要

Plant diseases caused by fungi, bacteria, and viruses pose a critical threat to global food security. Chemical pesticides are ineffective and might be harmful to the environment. Hence, there is an urgent need to develop alternative and eco-friendly strategies for managing plant diseases. Nanobiotechnology, particularly the use of silver nanoparticles (AgNPs), offers a promising solution. Antagonistic bacteria, due to their natural antimicrobial properties, can serve as effective agents for nanoparticle synthesis. This study investigates the green (bacteria-mediated) synthesis of AgNPs using Pseudomonas fluorescens as an eco-friendly approach to control Cochliobolus miyabeanus, the source of brown leaf in rice. The synthesis of AgNPs was facilitated by Pseudomonas fluorescens, utilizing bacterial biomolecules and enzymes to catalyze nanoparticle formation. FTIR analysis revealed the involvement of secondary metabolites, identified the functional group in the 3434–400 cm⁻1 range. The nanoparticles were further characterized using UV–Vis spectroscopy, SEM (revealing spherical, rod, and cubic shapes), and EDX (showing 32.93% silver content), with an average size of the particle is 50 nm. Morphological changes, such as hyphal tip swelling, bursting, and structural deformation observed in C. miyabeanus upon exposure to AgNPs, highlight their potent fungicidal activity, demonstrating the efficacy of green-synthesized AgNPs as sustainable biocontrol agents. Thus, Pseudomonas fluorescens emerges as a promising candidate for antifungal activity against brown leaf spot in rice, offering a viable, environmentally friendly alternative to chemical pesticides.