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<p>Chronic wound infections have significant global health implications, leading to complications, prolonged suffering, and, most times, death. These wounds are hard to manage because of the multidrug-resistant (MDR) bacteria that colonize them. There is a need to produce alternative therapeutics against these MDR bacteria. Microbes offer a robust eco-friendly source for assembling nanotherapeutics. Therefore, we produced gold nanoparticles (AuNPs) with the wet biomass of <i>Lysinibacillus fusiformis</i> (<i>L. fusiformis</i>) and 1&#xa0;mM HAuCl<sub>4</sub> solution. The AuNPs suspension synthesized was observed for color change, monitored by UV–vis spectroscopy, oven-dried, and characterized via SEM, EDX, DLS, TEM, XRD, and FTIR analyses. The AuNPs were assessed for their antibacterial potential against chronic wound bacteria by adopting a well-diffusion procedure, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. The cells of <i>L. fusiformis</i> optimally produced colloidal AuNPs with deep purple suspension and UV–vis λ<i>max</i> at 546&#xa0;nm. The AuNPs are distinctively spherical and possess 23.75% zerovalent gold, demonstrated by signals at 1.7&#xa0;keV and 2.2&#xa0;keV. The AuNPs have a mean size distribution of 121.167 ± 1.004&#xa0;nm, a PDI of 0.194 ± 0.009, but a mean distribution diameter of 18.6 ± 1.03&#xa0;nm (validated by TEM). The XRD showed distinct Bragg’s peaks of crystalline gold, while FTIR revealed the existence of methyl and amine groups. The AuNPs displayed potential antibacterial activities against MDR <i>Pseudomonas aeruginosa</i>, <i>Proteus mirabilis</i>, and <i>Enterobacter hormaechei</i> recovered from chronic wounds. This implies that the <i>L. fusiformis-</i>produced AuNPs possess pharmaceutical prospects for treating wound infections caused by MDR bacteria.</p> Graphical Abstract <p></p>

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Intracellular Production of Gold Nanoparticles from Lysinibacillus fusiformis, Their Spectro-Structural Characterization and Antibacterial Potential on MDR Bacteria of Chronic Wounds

  • 期刊论文

  • 发表日期：

    2025年3月24日

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  • Richard Kolade Omole,
  • Elizabeth Oladoyin Agboluaje,
  • Nkem Torimiro,
  • May P. Xiong,
  • Oluwole Isaac Adeyemi,
  • Reama Chinedu George,
  • Oluwafemi Bamidele Daramola,
  • Saravanan Muthupandian

摘要

Chronic wound infections have significant global health implications, leading to complications, prolonged suffering, and, most times, death. These wounds are hard to manage because of the multidrug-resistant (MDR) bacteria that colonize them. There is a need to produce alternative therapeutics against these MDR bacteria. Microbes offer a robust eco-friendly source for assembling nanotherapeutics. Therefore, we produced gold nanoparticles (AuNPs) with the wet biomass of Lysinibacillus fusiformis (L. fusiformis) and 1 mM HAuCl4 solution. The AuNPs suspension synthesized was observed for color change, monitored by UV–vis spectroscopy, oven-dried, and characterized via SEM, EDX, DLS, TEM, XRD, and FTIR analyses. The AuNPs were assessed for their antibacterial potential against chronic wound bacteria by adopting a well-diffusion procedure, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. The cells of L. fusiformis optimally produced colloidal AuNPs with deep purple suspension and UV–vis λmax at 546 nm. The AuNPs are distinctively spherical and possess 23.75% zerovalent gold, demonstrated by signals at 1.7 keV and 2.2 keV. The AuNPs have a mean size distribution of 121.167 ± 1.004 nm, a PDI of 0.194 ± 0.009, but a mean distribution diameter of 18.6 ± 1.03 nm (validated by TEM). The XRD showed distinct Bragg’s peaks of crystalline gold, while FTIR revealed the existence of methyl and amine groups. The AuNPs displayed potential antibacterial activities against MDR Pseudomonas aeruginosa, Proteus mirabilis, and Enterobacter hormaechei recovered from chronic wounds. This implies that the L. fusiformis-produced AuNPs possess pharmaceutical prospects for treating wound infections caused by MDR bacteria.

Graphical Abstract