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<p>Gold nanocages (AuNCs) exhibit tunable local surface plasmon resonance (LSPR) properties and exceptional drug loading and releasing capabilities, attributes stemming from their hollow interiors and ultra-thin porous walls. These features underscore their promising applications in the biomedical field. In this study, we fabricated AuNCs with LSPR peaks at 810&#xa0;nm via chemical etching of Ag nanocube templates with sharp edges using a precisely controlled volume of chloroauric acid. To impart gene-loading functionality, we chemically anchored thiol-modified polyethyleneimine (PEI) onto the surfaces of AuNCs through robust Au–S bonds, generating a novel gene carrier, AuNCs-PEI. Subsequently, we evaluated its photothermal efficacy using a femtosecond laser and assessed cytotoxicity via MTT assay. The binding affinity of AuNCs-PEI for EGFP plasmids was characterized by gel retardation assays. Furthermore, the gene delivery efficiency of AuNCs-PEI was examined in 293&#xa0;T cells by quantifying green fluorescence protein signals using inverted fluorescence microscopy. These findings demonstrate that engineered AuNCs-PEI not only facilitates gene loading for therapeutic applications but also enables photothermal therapy (PTT).</p>

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Engineering a Photothermally Responsive AuNCs-PEI Gene Carrier for Enhanced Gene Delivery

  • 期刊论文

  • 发表日期：

    2025年3月19日

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  • He-Qing Cai,
  • Lu Han,
  • Xin-Yu Xue,
  • Kou Zhang,
  • Tian-Hao Wang,
  • Fu-Hong Pan,
  • Zhi-Cheng Sun,
  • Ru-Ping Liu,
  • Kun Hu,
  • Yen Wei

摘要

Gold nanocages (AuNCs) exhibit tunable local surface plasmon resonance (LSPR) properties and exceptional drug loading and releasing capabilities, attributes stemming from their hollow interiors and ultra-thin porous walls. These features underscore their promising applications in the biomedical field. In this study, we fabricated AuNCs with LSPR peaks at 810 nm via chemical etching of Ag nanocube templates with sharp edges using a precisely controlled volume of chloroauric acid. To impart gene-loading functionality, we chemically anchored thiol-modified polyethyleneimine (PEI) onto the surfaces of AuNCs through robust Au–S bonds, generating a novel gene carrier, AuNCs-PEI. Subsequently, we evaluated its photothermal efficacy using a femtosecond laser and assessed cytotoxicity via MTT assay. The binding affinity of AuNCs-PEI for EGFP plasmids was characterized by gel retardation assays. Furthermore, the gene delivery efficiency of AuNCs-PEI was examined in 293 T cells by quantifying green fluorescence protein signals using inverted fluorescence microscopy. These findings demonstrate that engineered AuNCs-PEI not only facilitates gene loading for therapeutic applications but also enables photothermal therapy (PTT).