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<p>Osteosarcoma (OS) is the most common cancerous bone tumor in children and adolescents. By loading combretastatin A4 (CA4) onto the β-cyclodextrin-magnetic-graphene oxide (β-CD-MGO) nanoparticles decorated with folic acid (FA), β-CD-MGO-CA4-FA was created, and apoptotic and anti-metastatic effects on the MG-63 cell line were evaluated. β-CD-MGO-CA4-FA was assessed using DLS, FESEM, FTIR, XRD, and EDX for size distribution, morphology, structure, and elemental composition. MG-63 cells treated with β-CD-MGO-CA4-FA were evaluated through viability assays, cell cycle analysis, apoptosis assays, ROS generation, invasion assays, and migration wound healing. Additionally, mRNA and protein expressions related to apoptosis, metastasis, and MMP-2/MMP-9 activity were evaluated. The study characterizes the β-CD-MGO-CA4-FA nanocomposite, confirming functional groups, morphology, and iron conjugation, with a 41.3% encapsulation efficiency for CA4. Compared to CA4 alone, β-CD-MGO-CA4-FA more effectively reduced MG-63 cell viability, induced apoptosis, caused G2/M cell cycle arrest, elevated ROS production, decreased invasion, and modulated gene expression, leading to a more pronounced inhibition of MMP-2 and MMP-9 activities. Overall, β-CD-MGO-CA4-FA exhibits superior apoptotic and anti-metastatic effects compared to free CA4, possibly due to nanoparticle decorated with FA, improving cellular drug absorption.</p>

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Folate-Decorated β-Cyclodextrin-Magnetic-Graphene Oxide Nanoparticles Loaded with Combretastatin A4 for Osteosarcoma Cancer: an In Vitro and In Silico Study

  • 期刊论文

  • 发表日期：

    2025年3月14日

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  • Mehdi Rostami,
  • Elham Einafshar,
  • Maryam Shojaee,
  • Mohammad Soukhtanloo,
  • Mohammad Jalili-Nik

摘要

Osteosarcoma (OS) is the most common cancerous bone tumor in children and adolescents. By loading combretastatin A4 (CA4) onto the β-cyclodextrin-magnetic-graphene oxide (β-CD-MGO) nanoparticles decorated with folic acid (FA), β-CD-MGO-CA4-FA was created, and apoptotic and anti-metastatic effects on the MG-63 cell line were evaluated. β-CD-MGO-CA4-FA was assessed using DLS, FESEM, FTIR, XRD, and EDX for size distribution, morphology, structure, and elemental composition. MG-63 cells treated with β-CD-MGO-CA4-FA were evaluated through viability assays, cell cycle analysis, apoptosis assays, ROS generation, invasion assays, and migration wound healing. Additionally, mRNA and protein expressions related to apoptosis, metastasis, and MMP-2/MMP-9 activity were evaluated. The study characterizes the β-CD-MGO-CA4-FA nanocomposite, confirming functional groups, morphology, and iron conjugation, with a 41.3% encapsulation efficiency for CA4. Compared to CA4 alone, β-CD-MGO-CA4-FA more effectively reduced MG-63 cell viability, induced apoptosis, caused G2/M cell cycle arrest, elevated ROS production, decreased invasion, and modulated gene expression, leading to a more pronounced inhibition of MMP-2 and MMP-9 activities. Overall, β-CD-MGO-CA4-FA exhibits superior apoptotic and anti-metastatic effects compared to free CA4, possibly due to nanoparticle decorated with FA, improving cellular drug absorption.