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<p>In this in vitro study, nanochitosan was synthesized from the cuttlebones of <i>Sepia kobiensis</i> and characterized for its potential cytotoxicity and antimicrobial applications in dentistry. The synthesis involved using sodium tripolyphosphate solution with continuous stirring. The resulting nanoparticles were structurally characterized by FTIR, SEM, and XRD analyses, revealing their structural properties. The nanoparticles, ranging in size from 73 to 151&#xa0;nm, were spherical and exhibited positive surface charges. Antimicrobial tests showed that nanochitosan effectively inhibited dental pathogens such as <i>Candida albicans</i> (25 ± 2.2&#xa0;mm) and <i>Escherichia coli</i> (23 ± 1.7&#xa0;mm). The nanoparticles adhered to bacterial cell surfaces, disrupting their membranes and causing cell death. Additionally, nanochitosan demonstrated biocompatibility with human gingival fibroblasts and showed potential cytotoxic activity by inducing oxidative stress and apoptosis in cancer cells. These findings suggest that nanochitosan derived from <i>S. kobiensis</i> cuttlebone holds significant promise as a multifunctional agent in dental healthcare, offering an innovative approach to combating dental infections and potentially contributing to oral cancer therapies.</p>

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Harnessing Nanochitosan from Sepia kobiensis Cuttlebone for Cytotoxicity and Antimicrobial Applications in Dentistry: An In Vitro Study

  • 期刊论文

  • 发表日期：

    2025年2月4日

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  • B. Sabnam Ali,
  • Namasivayam Subhapradha,
  • Annathai Pitchai,
  • Pasiyappazham Ramasamy

摘要

In this in vitro study, nanochitosan was synthesized from the cuttlebones of Sepia kobiensis and characterized for its potential cytotoxicity and antimicrobial applications in dentistry. The synthesis involved using sodium tripolyphosphate solution with continuous stirring. The resulting nanoparticles were structurally characterized by FTIR, SEM, and XRD analyses, revealing their structural properties. The nanoparticles, ranging in size from 73 to 151 nm, were spherical and exhibited positive surface charges. Antimicrobial tests showed that nanochitosan effectively inhibited dental pathogens such as Candida albicans (25 ± 2.2 mm) and Escherichia coli (23 ± 1.7 mm). The nanoparticles adhered to bacterial cell surfaces, disrupting their membranes and causing cell death. Additionally, nanochitosan demonstrated biocompatibility with human gingival fibroblasts and showed potential cytotoxic activity by inducing oxidative stress and apoptosis in cancer cells. These findings suggest that nanochitosan derived from S. kobiensis cuttlebone holds significant promise as a multifunctional agent in dental healthcare, offering an innovative approach to combating dental infections and potentially contributing to oral cancer therapies.