Synthesis of the Cobalt Polyacrylate (Hestatin) and Testing of Its Haemostatic Properties
摘要
A cobalt polyacrylate tight complex was synthesized from polyacrylic acid (PAA) with average molecular masses of 9356 and 184,631 kDa. The source PAA with programmed molecular weight was synthesized under controlled conditions using isopropanol as a chain growth controlling agent. Tight complexes with Co2+ yielded 1:32 PAA units in the case of 10 kDa (Hestatin 10) and 1:19 in the case of 200 kDa (Hestatin 200). Purification of the tight complexes from the Co2+ in the weakly bound salt form was carried out by using sequential dialysis against deionized water. Testing of the haemostatic activity of the manufactured tight complexes on an animal model of capillary and venous bleeding was carried out; free PAA 10 and PAA 200 were used as the control samples. The experiment demonstrated high haemostatic efficacy of Hestatin 200, Hestatin 10, and PAA 10, whereas PAA 200 acted as an antithrombotic agent.