Scholarlink

Introduction <p><i>Fusobacterium Nucleatum</i> an oral pathogen associated with periodontal disease, has been recently linked to etiopathogenesis of carcinoma, due to its ability to evade immune surveillance, promote inflammation, and facilitate cellular proliferation highlighting its potential role in the development and progression of malignancies. In the study we explored the occurrence of <i>Fusobacterium nucleatum</i> in patients with head and neck cancer (HNC) and the relationship between its DNA status and various clinicopathological features.</p> Materials and Methods <p>A cohort of 100 HNC patients who underwent biopsy at the Department of Oral and Maxillofacial Surgery, King George Medical University, was analysed. Both cancerous and adjacent normal tissue samples were collected, and <i>F. nucleatum</i> DNA levels were quantified using quantitative PCR (qPCR). Relationships between <i>F. nucleatum</i> positivity and quantity with demographic and clinicopathological parameters (tumor differentiation, size, locoregional lymph node status, distant metastasis) were assessed.</p> Results <p><i>F. nucleatum</i> was meaningfully overrepresented in cancer biopsy tissues in comparison to adjacent normal tissue, with a median fold change of 4.41 (IQR=0.87-41.66) in tumor samples versus 1 (IQR=1-1.02) in normal tissue. The bacterial presence was associated with tumor size (T stage) but not with nodal stage or tumor differentiation. However, <i>F. nucleatum</i> positivity was not identified as a risk factor for cancer severity (stages III and IV).</p> Conclusion <p>The study highlights the significant abundance of <i>F. nucleatum</i>, suggesting its possible role as a biomarker for HNC. Future studies are warranted to assess its prognostic value and explore therapeutic interventions. </p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Detection and Relative Quantification of Fusobacterium Nucleatum in Head and Neck Cancers

  • 期刊论文

  • 发表日期：

    2026年2月3日

查看全文
  • Sumedha Mohan Popli,
  • Uma Shanker Pal,
  • Vimala Venkatesh,
  • Piyush Tripathi,
  • Anuj Verma,
  • Gourav Popli,
  • Yash Merchant

摘要

Introduction

Fusobacterium Nucleatum an oral pathogen associated with periodontal disease, has been recently linked to etiopathogenesis of carcinoma, due to its ability to evade immune surveillance, promote inflammation, and facilitate cellular proliferation highlighting its potential role in the development and progression of malignancies. In the study we explored the occurrence of Fusobacterium nucleatum in patients with head and neck cancer (HNC) and the relationship between its DNA status and various clinicopathological features.

Materials and Methods

A cohort of 100 HNC patients who underwent biopsy at the Department of Oral and Maxillofacial Surgery, King George Medical University, was analysed. Both cancerous and adjacent normal tissue samples were collected, and F. nucleatum DNA levels were quantified using quantitative PCR (qPCR). Relationships between F. nucleatum positivity and quantity with demographic and clinicopathological parameters (tumor differentiation, size, locoregional lymph node status, distant metastasis) were assessed.

Results

F. nucleatum was meaningfully overrepresented in cancer biopsy tissues in comparison to adjacent normal tissue, with a median fold change of 4.41 (IQR=0.87-41.66) in tumor samples versus 1 (IQR=1-1.02) in normal tissue. The bacterial presence was associated with tumor size (T stage) but not with nodal stage or tumor differentiation. However, F. nucleatum positivity was not identified as a risk factor for cancer severity (stages III and IV).

Conclusion

The study highlights the significant abundance of F. nucleatum, suggesting its possible role as a biomarker for HNC. Future studies are warranted to assess its prognostic value and explore therapeutic interventions.