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Purpose <p>This study aimed to compare the bone regenerative effects of different doses of strontium ranelate (SR) with those of BMP-2, by applying collagen sponges (CS) absorbed with two concentrations of SR and BMP-2 to a rat calvarial defect model and evaluating the outcomes through histological and immunohistochemical analyses.</p> Material and Methods <p>Critical-sized calvarial defects created on 34 male Long Evans rats. The defects were left empty or filled with only CS, CS absorbed with 0.25&#xa0;mM SR (CS + 0.25 SR), CS absorbed with 0.5&#xa0;mM SR (CS + 0.5 SR), and CS absorbed with 0.1&#xa0;mg/ml rHBMP-2 (CS + BMP-2). Samples taken at the end of 8&#xa0;weeks post-operation were examined histologically and immunohistochemically.</p> Results <p>There was no significant difference in the percentage of new bone formation (NBF) between the CS and SR groups, while the BMP-2 group showed a higher NBF level than the CS. There was no significant difference in the percentage of NBF between the 0.25 SR group and the BMP-2 group (<i>p</i> &lt; 0.001). In osteocalcin analysis, the BMP-2 group exhibited a higher immune positive compared to the other groups.</p> Conclusion <p>0.25&#xa0;mM SR showed comparable osteogenic potential to BMP-2, while the higher 0.5&#xa0;mM SR dose was ineffective, possibly due to carrier limitations or dose-related suppression of bone formation.</p>

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Local Application of Strontium Ranelate and BMP-2 in Bone Regeneration: A Dose-Dependent Study in a Rat Calvarial Defect Model

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Işıl Karapınar,
  • Altan Doğan,
  • Sühan Gürbüz,
  • Fatma Nur Tuğcu Demiröz,
  • Zeynep Yığman,
  • Suna Ömeroğlu

摘要

Purpose

This study aimed to compare the bone regenerative effects of different doses of strontium ranelate (SR) with those of BMP-2, by applying collagen sponges (CS) absorbed with two concentrations of SR and BMP-2 to a rat calvarial defect model and evaluating the outcomes through histological and immunohistochemical analyses.

Material and Methods

Critical-sized calvarial defects created on 34 male Long Evans rats. The defects were left empty or filled with only CS, CS absorbed with 0.25 mM SR (CS + 0.25 SR), CS absorbed with 0.5 mM SR (CS + 0.5 SR), and CS absorbed with 0.1 mg/ml rHBMP-2 (CS + BMP-2). Samples taken at the end of 8 weeks post-operation were examined histologically and immunohistochemically.

Results

There was no significant difference in the percentage of new bone formation (NBF) between the CS and SR groups, while the BMP-2 group showed a higher NBF level than the CS. There was no significant difference in the percentage of NBF between the 0.25 SR group and the BMP-2 group (p < 0.001). In osteocalcin analysis, the BMP-2 group exhibited a higher immune positive compared to the other groups.

Conclusion

0.25 mM SR showed comparable osteogenic potential to BMP-2, while the higher 0.5 mM SR dose was ineffective, possibly due to carrier limitations or dose-related suppression of bone formation.