Abstract <p>Over the past two decades, light sheet fluorescence microscopy has developed to a powerful tool for studies of dynamics in biological systems. In a new development, we apply light sheet fluorescence microscopy as a novel experimental measurement technology within the field of multiphase process engineering and fluid dynamics. The technology enables a number of novel studies of single- and multiphase transport phenomena on a microscopic scale. In the current publication, we introduce the first implementation of light sheet fluorescence microscopy in the field and demonstrate its applicability on one exemplary measurement of diffusive oxygen mass transfer from an oxygen bubble to degassed water. The results prove that such measurements can be conducted with a high spatial resolution with a submicron pixel pitch and enable precise studies on microscopic transport phenomena. Besides measurements of mass transfer, on which we lay the focus here, light sheet fluorescence microscopy further enables studies of fluid dynamics on a microscopic scale.</p> Graphical abstract <p></p>