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<p>Microbial degradation strategy is one of the most beneficial methods to convert an organic biomass into valuable products. In this study, bioethanol can be produced with the help of microbes isolated from the gut of wood borer larvae <i>Oryctes</i> <i>rhinoceros</i>. Isolate and characterization of microbes and their cellulolytic ability were carried out. Two microbes from <i>O.</i> <i>rhinoceros</i> have cellulolytic activity and belong to the genes <i>Enterobacter sp</i> and <i>Klebsiella</i> <i>sp.</i> These two microbes are used in the hydrolysis process of rice straw and banana leaf wastes. The concentration of reducing sugars can be analysed by UV–Vis Spectrophotometer. The hydrolysed samples were kept for the fermentation process. And the functional group of the fermented samples was analysed using FTIR analysis, which confirmed the presence of an OH group in the sample. The qualitative analysis of reducing sugar released from the cellulolytic bacteria using Benedict’s test, which produce a red colour precipitate conforms to the presence of reducing sugar. The quantitative analysis of reducing sugar using DNSA method indicates a dark brown colour, On the 9th days of hydrolysis by <i>Enterobacter sp, Klebsiella sp</i> and combination of <i>Enterobacter sp</i>, <i>Klebsiella sp</i>, it can be seen that the reducing sugar can be obtained in the amounts of 1.35&#xa0;mg/ml 1.22&#xa0;mg/ml and 1.48&#xa0;mg/ml, respectively, in the microbial treated samples when compared with the control. Based on the results, I concluded that the second-generation bioethanol can be produced from the agricultural wastes using the microbes isolated from the gut of <i>Oryctes</i> <i>rhinoceros</i> larvae. And evaluate the mosquitocidal activity of secondary metabolites from those bacteria against <i>C.</i> <i>quinquefasciatus</i> larvae, which shows that LC<sub>50</sub> of the ethyl acetate extract of <i>Enterobacter</i> <i>sp</i> was 480.003&#xa0;ppm and LC<sub>90</sub> was 1709.205&#xa0;ppm and they LC<sub>50</sub> of <i>Klebsiella</i> <i>sp</i> are 276.911&#xa0;ppm and LC<sub>90</sub> is 1090.845&#xa0;ppm at 72h of exposure. So, the study concluded that they are non-toxic to humans, it also reduces the use of fossil fuels and helps in the management of agricultural waste.</p> Graphical Abstract <p></p>

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Isolation of Cellulolytic Microbes From the Gut of Wood Borer (Oryctes Rhinoceros) for the Conversion of Biomass and Production of Bioethanol, and Evaluating Their Mosquitocidal Efficacy

  • 期刊论文

  • 发表日期：

    2026年3月16日

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  • Thamaraikkannan Srisangamesh,
  • Shankar Santhoshkumar,
  • Kasilingam Balumahendhiran,
  • Muthugounder Subramanian Shivakumar

摘要

Microbial degradation strategy is one of the most beneficial methods to convert an organic biomass into valuable products. In this study, bioethanol can be produced with the help of microbes isolated from the gut of wood borer larvae Oryctes rhinoceros. Isolate and characterization of microbes and their cellulolytic ability were carried out. Two microbes from O. rhinoceros have cellulolytic activity and belong to the genes Enterobacter sp and Klebsiella sp. These two microbes are used in the hydrolysis process of rice straw and banana leaf wastes. The concentration of reducing sugars can be analysed by UV–Vis Spectrophotometer. The hydrolysed samples were kept for the fermentation process. And the functional group of the fermented samples was analysed using FTIR analysis, which confirmed the presence of an OH group in the sample. The qualitative analysis of reducing sugar released from the cellulolytic bacteria using Benedict’s test, which produce a red colour precipitate conforms to the presence of reducing sugar. The quantitative analysis of reducing sugar using DNSA method indicates a dark brown colour, On the 9th days of hydrolysis by Enterobacter sp, Klebsiella sp and combination of Enterobacter sp, Klebsiella sp, it can be seen that the reducing sugar can be obtained in the amounts of 1.35 mg/ml 1.22 mg/ml and 1.48 mg/ml, respectively, in the microbial treated samples when compared with the control. Based on the results, I concluded that the second-generation bioethanol can be produced from the agricultural wastes using the microbes isolated from the gut of Oryctes rhinoceros larvae. And evaluate the mosquitocidal activity of secondary metabolites from those bacteria against C. quinquefasciatus larvae, which shows that LC50 of the ethyl acetate extract of Enterobacter sp was 480.003 ppm and LC90 was 1709.205 ppm and they LC50 of Klebsiella sp are 276.911 ppm and LC90 is 1090.845 ppm at 72h of exposure. So, the study concluded that they are non-toxic to humans, it also reduces the use of fossil fuels and helps in the management of agricultural waste.

Graphical Abstract