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<p>This study aims to streamline the preparation and compositional qualities of fish silage, with special interest given to the processing parameters and their significance for industrial uses. The study examines the effect of temperature and agitation speed (RPM) on optimising the production of amino acids and proteins in Biological Fish Silage (BFS) and Acid Fish Silage (AFS). Efficiency, trace metal profiles, and nutrient density of different formulations are also studied, and use of molasses as an alternative to traditional acidification techniques is investigated in the study. Findings show that 30&#xa0;°C is the optimal temperature for protein synthesis in both AFS and BFS, where protein concentration is significantly higher on days 3 and 4 compared to 20&#xa0;°C and 40&#xa0;°C. The agitation of 150 RPM facilitates the steady production of individual amino acids, while 100 RPM displays an initial increase and subsequent decrease, and agitation at 200 RPM exhibits pronounced oscillations. The trace metal analysis reveals that AFS contains more calcium, sodium, and potassium, while BFS contains more potassium. BFS developed using molasses has a better nutritional value and environmental properties, providing an environmentally friendly and cost-effective alternative to aquaculture feed, and may also be used as a biofertiliser.</p> Graphic Abstract <p></p>

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From Waste to Wealth: Optimizing Fish Silage for Aquafeed and Environmental Impact

  • 期刊论文

  • 发表日期：

    2026年3月5日

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  • Kirtani Palekar,
  • Rakhee D. S. Khandeparker,
  • Rishi Gandhi,
  • Adhira Mahale

摘要

This study aims to streamline the preparation and compositional qualities of fish silage, with special interest given to the processing parameters and their significance for industrial uses. The study examines the effect of temperature and agitation speed (RPM) on optimising the production of amino acids and proteins in Biological Fish Silage (BFS) and Acid Fish Silage (AFS). Efficiency, trace metal profiles, and nutrient density of different formulations are also studied, and use of molasses as an alternative to traditional acidification techniques is investigated in the study. Findings show that 30 °C is the optimal temperature for protein synthesis in both AFS and BFS, where protein concentration is significantly higher on days 3 and 4 compared to 20 °C and 40 °C. The agitation of 150 RPM facilitates the steady production of individual amino acids, while 100 RPM displays an initial increase and subsequent decrease, and agitation at 200 RPM exhibits pronounced oscillations. The trace metal analysis reveals that AFS contains more calcium, sodium, and potassium, while BFS contains more potassium. BFS developed using molasses has a better nutritional value and environmental properties, providing an environmentally friendly and cost-effective alternative to aquaculture feed, and may also be used as a biofertiliser.

Graphic Abstract