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Purpose <p>This study reports a promising mutant strain of <i>Pseudomonas monteilii</i> with enhanced polyhydroxyalkanoate (PHA) production from crude glycerol.</p> Methods <p>The strain was developed using a combined approach of random UV mutagenesis and flow cytometric cell sorting (FACS) to rapidly select for PHA-overproducing mutant. The isolated mutant strain designated as MT-gly-13 was capable of accumulating PHA at 156.0 ± 2.6 mg/L in batch shake flask experiment containing 20 g/l crude glycerol, 1.84-fold higher than the wild-type.</p> Results <p>The PHA production was further increased through optimizing media composition in batch and varying pulse feeding profiles in fed-batch experiments, which led to PHA titer of 530.6 ± 4.4 mg/L and PHA content of 8.35% cell dry weight. PHA is composed of74.5% 3HB, 0.4% 3HHx, 4.7% 3HO and 20.4% 3HD based on GC–MS analysis.</p> Conclusion <p>The results demonstrated the potential utilization of crude glycerol to produce added-value byproducts such as bioplastics along with biodiesel which could further support the economic and sustainable development of the biodiesel industry.</p> Graphical Abstract <p></p>

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Optimizing Crude Glycerol to Bioplastics by a Mutant Strain of Pseudomonas monteilii

  • 期刊论文

  • 发表日期：

    2026年2月9日

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  • Punpaporn Ketsakhon,
  • Napaporn Sittirangsinan,
  • Wuttichai Mhuantong,
  • Pornkamol Unrean,
  • Verawat Champreda

摘要

Purpose

This study reports a promising mutant strain of Pseudomonas monteilii with enhanced polyhydroxyalkanoate (PHA) production from crude glycerol.

Methods

The strain was developed using a combined approach of random UV mutagenesis and flow cytometric cell sorting (FACS) to rapidly select for PHA-overproducing mutant. The isolated mutant strain designated as MT-gly-13 was capable of accumulating PHA at 156.0 ± 2.6 mg/L in batch shake flask experiment containing 20 g/l crude glycerol, 1.84-fold higher than the wild-type.

Results

The PHA production was further increased through optimizing media composition in batch and varying pulse feeding profiles in fed-batch experiments, which led to PHA titer of 530.6 ± 4.4 mg/L and PHA content of 8.35% cell dry weight. PHA is composed of74.5% 3HB, 0.4% 3HHx, 4.7% 3HO and 20.4% 3HD based on GC–MS analysis.

Conclusion

The results demonstrated the potential utilization of crude glycerol to produce added-value byproducts such as bioplastics along with biodiesel which could further support the economic and sustainable development of the biodiesel industry.

Graphical Abstract