Scholarlink

Purpose <p>This comparative study aims to explore the potential of fish scale collagen for biomedical application by evaluating the yield and characteristics of collagen extracted from the scales of three carps by acid and pepsin solubilization methods.</p> Methods and Results <p>The collagen yield varied among three species and extraction methods, with the average value of 0.53% (ASC) and 0.91% (PSC). The FTIR results confirmed the successful formation of collagen with the presence of characteristic amide peaks (I:1633–1637&#xa0;cm⁻¹, II:1545–1546&#xa0;cm⁻¹, III:1234–1238&#xa0;cm⁻¹). The FESEM analysis revealed that the ASC exhibited a well-organized fibrous network with fibre diameters of 2.65 ± 1.02&#xa0;μm to 3.94 ± 2.23&#xa0;μm, while the PSC showed loosely arranged fibres with thinner diameters of 1.809 ± 0.568&#xa0;μm to 2.17 ± 0.96&#xa0;μm. The TGA analysis demonstrated that the ASC had a higher thermal decomposition than the PSC. The presence of two α (130&#xa0;kDa) and one β (180&#xa0;kDa) chain in SDS-PAGE results also confirmed the presence of Type-I collagen among species. Glycine was the dominant amino acid (31.6%) while proline (0.94%) was comparatively low. Following comparative analysis, grass carp collagen was selected for further evaluation for its biocompatibility and angiogenic potential through <i>in ovo</i> chorioallantoic membrane (CAM) assay.</p> Conclusion <p>These findings suggest that the PSC showed better yield than the ASC, with minor differences in thermal stability and morphological characteristics. The approach provides ecological benefits by mitigating biowaste accumulation and offers economic incentives for small-scale fisheries through value-added product generation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fish Scale Waste Valorization for High-Value Biomaterial: Comparative Analysis of Type I Collagen from Three Carp Species by Acid and Pepsin Solubilization Methods

  • 期刊论文

  • 发表日期：

    2025年12月9日

查看全文
  • Bandu Matiyal,
  • Rishu Dalal,
  • Aisha Jamal,
  • Chhavi Chawla,
  • Diya Verma,
  • Ravneet Kaur

摘要

Purpose

This comparative study aims to explore the potential of fish scale collagen for biomedical application by evaluating the yield and characteristics of collagen extracted from the scales of three carps by acid and pepsin solubilization methods.

Methods and Results

The collagen yield varied among three species and extraction methods, with the average value of 0.53% (ASC) and 0.91% (PSC). The FTIR results confirmed the successful formation of collagen with the presence of characteristic amide peaks (I:1633–1637 cm⁻¹, II:1545–1546 cm⁻¹, III:1234–1238 cm⁻¹). The FESEM analysis revealed that the ASC exhibited a well-organized fibrous network with fibre diameters of 2.65 ± 1.02 μm to 3.94 ± 2.23 μm, while the PSC showed loosely arranged fibres with thinner diameters of 1.809 ± 0.568 μm to 2.17 ± 0.96 μm. The TGA analysis demonstrated that the ASC had a higher thermal decomposition than the PSC. The presence of two α (130 kDa) and one β (180 kDa) chain in SDS-PAGE results also confirmed the presence of Type-I collagen among species. Glycine was the dominant amino acid (31.6%) while proline (0.94%) was comparatively low. Following comparative analysis, grass carp collagen was selected for further evaluation for its biocompatibility and angiogenic potential through in ovo chorioallantoic membrane (CAM) assay.

Conclusion

These findings suggest that the PSC showed better yield than the ASC, with minor differences in thermal stability and morphological characteristics. The approach provides ecological benefits by mitigating biowaste accumulation and offers economic incentives for small-scale fisheries through value-added product generation.