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<p>Recently, efforts have been made to move the fishing industry towards smart fishing, enabling the long-term sustainability of the activity. Fish enzymes from viscera could be recycled and valorised for detergent use. This study aims to promote the application of a circular economy model using viscera from the southern long-tail cod (<i>Patagonotothen ramsayi</i>) fishery. Crude extracts of stomachs and intestines were purified using ammonium sulphate followed by membrane ultrafiltration. A full factorial design was used to select the conditions for the highest enzymatic activity, resulting in pH 8, 45&#xa0;°C for 15&#xa0;min and pH 2, 50&#xa0;°C for 20&#xa0;min for alkaline and acid proteases respectively. All activities were enhanced by the presence of CaCl<sub>2</sub>, Triton X-100, Tween 20 and Tween 80. However, SDS only had a positive effect on stomach proteinases. Acidic enzymes were stable in the presence of all surfactants tested, but their activity was not significantly improved. Conversely, the activity of all extracts was partially inhibited by EDTA. Compatibility with commercial detergents and washing tests performed showed positive effects. The information obtained from this work will increase the knowledge of the enzymes of this fishery to be used as an ingredient, which could have a triple impact benefit.</p> Graphical Abstract <p></p>

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Recovery, Characterization and Potential Application as a Detergent Additive of Viscera Proteases from Longtail Southern Cod Patagonotothen ramsayi: A Transition to Circular Economy

  • 期刊论文

  • 发表日期：

    2025年10月31日

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  • Daniela Lorena Lamas,
  • Victoria Florencia Alcolea Ersinger,
  • Águeda Elena Massa

摘要

Recently, efforts have been made to move the fishing industry towards smart fishing, enabling the long-term sustainability of the activity. Fish enzymes from viscera could be recycled and valorised for detergent use. This study aims to promote the application of a circular economy model using viscera from the southern long-tail cod (Patagonotothen ramsayi) fishery. Crude extracts of stomachs and intestines were purified using ammonium sulphate followed by membrane ultrafiltration. A full factorial design was used to select the conditions for the highest enzymatic activity, resulting in pH 8, 45 °C for 15 min and pH 2, 50 °C for 20 min for alkaline and acid proteases respectively. All activities were enhanced by the presence of CaCl2, Triton X-100, Tween 20 and Tween 80. However, SDS only had a positive effect on stomach proteinases. Acidic enzymes were stable in the presence of all surfactants tested, but their activity was not significantly improved. Conversely, the activity of all extracts was partially inhibited by EDTA. Compatibility with commercial detergents and washing tests performed showed positive effects. The information obtained from this work will increase the knowledge of the enzymes of this fishery to be used as an ingredient, which could have a triple impact benefit.

Graphical Abstract