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Purpose <p>The study aims to evaluate the fermentation capabilities of <i>Pichia kudriavzevii</i> SVMS2019 using glucose, xylose, and their mixtures, as well as alkali-pretreated wheat straw biomass.</p> Methods <p>The fermentation optimization involved varying pH (2.0–7.0), incubation time (24–72&#xa0;h), temperature (30, 37, and 42 ℃), cell concentration (1–15%), pre-inoculum time (6–48&#xa0;h), and sugar mixture ratios (glucose: xylose from 0:10 to 10:0) using glucose, xylose, and glucose–xylose mixtures. Followed by 2% NaOH-pretreated wheat straw under separate hydrolysis and fermentation (SHF) method, simultaneous saccharification and fermentation (SSF) method was conducted.</p> Results <p>Optimal conditions for glucose: pH 5.0, 42 ℃, 72&#xa0;h incubation, 2% cell concentration, and 18&#xa0;h pre-inoculum. For xylose, the ideal conditions were pH 5.0, 30 ℃, 72&#xa0;h incubation, 5% cell concentration, and 18&#xa0;h pre-inoculum. Co-fermentation shared these conditions: pH 5.0, 42 ℃, 72&#xa0;h incubation, 5% cell concentration, and 18&#xa0;h pre-inoculum. High ethanol yields were observed with high proportion of xylose (1:9 and 2:8–glucose: xylose) in mixed sugar ratios. The enzyme cocktail achieved 94.3% efficiency, yielding 24.16&#xa0;g/L ethanol at 42 ℃ under SHF. Meanwhile, under SSF with <i>P. kudriavzevii</i> SVMS2019, the enzyme cocktail produced 22.44&#xa0;g/L ethanol at 42 ℃ with 95.63% efficiency.</p> Conclusion <p>This study contributes developing an industrial strain for lignocellulosic-based biorefinery processes, addressing energy needs.</p> Graphical Abstract <p></p>

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Enhanced Non-Conventional Yeast Pichia kudriavzevii SVMS2019 Fermentation Efficiencies with Hexose, Pentose and Classic Pretreated Wheatstraw Biomass

  • 期刊论文

  • 发表日期：

    2025年8月2日

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  • Merlin Sobia Poomani,
  • Iyyadurai Mariappan,
  • Karthik Balasubramanian,
  • Krishnaveni Muthan,
  • Venkatesh Subramanian

摘要

Purpose

The study aims to evaluate the fermentation capabilities of Pichia kudriavzevii SVMS2019 using glucose, xylose, and their mixtures, as well as alkali-pretreated wheat straw biomass.

Methods

The fermentation optimization involved varying pH (2.0–7.0), incubation time (24–72 h), temperature (30, 37, and 42 ℃), cell concentration (1–15%), pre-inoculum time (6–48 h), and sugar mixture ratios (glucose: xylose from 0:10 to 10:0) using glucose, xylose, and glucose–xylose mixtures. Followed by 2% NaOH-pretreated wheat straw under separate hydrolysis and fermentation (SHF) method, simultaneous saccharification and fermentation (SSF) method was conducted.

Results

Optimal conditions for glucose: pH 5.0, 42 ℃, 72 h incubation, 2% cell concentration, and 18 h pre-inoculum. For xylose, the ideal conditions were pH 5.0, 30 ℃, 72 h incubation, 5% cell concentration, and 18 h pre-inoculum. Co-fermentation shared these conditions: pH 5.0, 42 ℃, 72 h incubation, 5% cell concentration, and 18 h pre-inoculum. High ethanol yields were observed with high proportion of xylose (1:9 and 2:8–glucose: xylose) in mixed sugar ratios. The enzyme cocktail achieved 94.3% efficiency, yielding 24.16 g/L ethanol at 42 ℃ under SHF. Meanwhile, under SSF with P. kudriavzevii SVMS2019, the enzyme cocktail produced 22.44 g/L ethanol at 42 ℃ with 95.63% efficiency.

Conclusion

This study contributes developing an industrial strain for lignocellulosic-based biorefinery processes, addressing energy needs.

Graphical Abstract