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Purpose <p>The study aimed to utilize wheat and rice bran for developing an economic liquid medium for the production of <i>Azospirillum brasilense</i> liquid biofertilizer.</p> Methods and Results <p>The study aimed to develop a cost-effective liquid formulation for <i>Azospirillum brasilense</i> using wheat and rice bran. Evaluation of wheat and rice bran-based media formulations (1:10, 2:10, 3:10, 4:10, 5:10 and 6:10 with distilled water), for <i>A. brasilense</i> growth, showed progressive increase in cell viability with rising substrate concentration, peaking at 4:10 ratio with distilled water. Wheat (WB4) and rice bran (RB4) based bioformulations achieved maximum growth of 11.29 ± 0.08 and 11.28 ± 0.19 Log CFU/ml at 96&#xa0;h of incubation followed by stable growth. Assessment of trehalose (5, 10 and 15&#xa0;mM), carboxymethyl cellulose (CMC) (0.05 and 0.1%) and glycerol (0.5 and 1.0%) in WB4 and RB4 formulations proved 10&#xa0;mM trehalose supplementation to be most effective in maintaining highest cell viability and plant growth promoting traits up to 240&#xa0;days. Gnotobiotic and pot studies further demonstrated that seed biopriming with WB4 + 10&#xa0;mM trehalose-based <i>A. brasilense</i> bioformulation induced maximum seedling emergence and plant growth in rice (PR 126) compared to uninoculated control and even charcoal-based <i>A. brasilense</i> biofertilizer.</p> Conclusion <p>The WB4 bioformulation amended with 10&#xa0;mM trehalose sustained peak growth, shelf life and multifunctional traits of <i>A. brasilense</i> for eight months (240&#xa0;days), highlighting its potential as an economical substitute for expensive synthetic media, addressing a key challenge in the commercial production of biofertilizers.</p> Graphical Abstract <p></p>

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Cereal Bran Based Cost Effective Liquid Azospirillum brasilense Biofertilizer with Extended Growth, Multifunctional Attributes and Shelf Life

  • 期刊论文

  • 发表日期：

    2025年7月23日

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  • Priyanka Sharda,
  • Suman Kumari,
  • Neemisha

摘要

Purpose

The study aimed to utilize wheat and rice bran for developing an economic liquid medium for the production of Azospirillum brasilense liquid biofertilizer.

Methods and Results

The study aimed to develop a cost-effective liquid formulation for Azospirillum brasilense using wheat and rice bran. Evaluation of wheat and rice bran-based media formulations (1:10, 2:10, 3:10, 4:10, 5:10 and 6:10 with distilled water), for A. brasilense growth, showed progressive increase in cell viability with rising substrate concentration, peaking at 4:10 ratio with distilled water. Wheat (WB4) and rice bran (RB4) based bioformulations achieved maximum growth of 11.29 ± 0.08 and 11.28 ± 0.19 Log CFU/ml at 96 h of incubation followed by stable growth. Assessment of trehalose (5, 10 and 15 mM), carboxymethyl cellulose (CMC) (0.05 and 0.1%) and glycerol (0.5 and 1.0%) in WB4 and RB4 formulations proved 10 mM trehalose supplementation to be most effective in maintaining highest cell viability and plant growth promoting traits up to 240 days. Gnotobiotic and pot studies further demonstrated that seed biopriming with WB4 + 10 mM trehalose-based A. brasilense bioformulation induced maximum seedling emergence and plant growth in rice (PR 126) compared to uninoculated control and even charcoal-based A. brasilense biofertilizer.

Conclusion

The WB4 bioformulation amended with 10 mM trehalose sustained peak growth, shelf life and multifunctional traits of A. brasilense for eight months (240 days), highlighting its potential as an economical substitute for expensive synthetic media, addressing a key challenge in the commercial production of biofertilizers.

Graphical Abstract