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<p>The synthesis of second-generation bioethanol from sugarcane bagasse (SCB) is a promising method for reducing greenhouse gas emissions. The second generation of bioethanol production from SCB is investigated using batch anaerobic fermentation with microorganisms <i>Saccharomyces cerevisiae</i> (SC) and <i>Bacillus subtilis</i> (BS) along with a pre-treatment of basic hydrogen peroxide. SC has been used as the primary ethanol producer in industries because this microbe has a high tolerance for ethanol production. This property makes SC ideal for the production of ethanol in this process. The presence of BS does not directly play a part in ethanol production, but this microbe is used owing to its property of increased alcohol production from cellulose which is sugarcane bagasse in this case. The pretreatment conditions of alkaline hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) were optimized to the values 3&#xa0;h, 50&#xa0;°C, 60 psi, pH 8.6, and 150&#xa0;rpm. The analysis indicates improved production through batch fermentation pretreatment at various operational stages. After pretreatment, different qualitative characterization techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), and Thermogravimetric Analysis (TGA) results confirmed the better effects of structural changes of hemicellulose, lignin, and cellulose, mass loss, thermal degradation, and higher concentration of bioethanol in the distillate. The conversion of biomass to bioethanol using SC yields a high production yield (70%), with a production of 70&#xa0;g/L from 100&#xa0;g of SCB after 72&#xa0;h and a bioethanol yield (0.7&#xa0;g/g) of SCB confirmed through quantitative analysis from gas chromatography/mass spectrometry (GC/MS) and High-Performance Liquid Chromatography (HPLC).</p> Graphical Abstract <p></p>

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Impact of Pressure on the Alkaline Pretreated Sugarcane Bagasse Feedstock for the Enhanced Production of Bioethanol Through Fermentation Process

  • 期刊论文

  • 发表日期：

    2025年7月3日

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  • Azhar Uddin,
  • Rabia Liaquat,
  • Sadia Mumtaz,
  • Muhammad Muddasar,
  • Sami Ullah,
  • Abdul Basit,
  • Manal Arshad

摘要

The synthesis of second-generation bioethanol from sugarcane bagasse (SCB) is a promising method for reducing greenhouse gas emissions. The second generation of bioethanol production from SCB is investigated using batch anaerobic fermentation with microorganisms Saccharomyces cerevisiae (SC) and Bacillus subtilis (BS) along with a pre-treatment of basic hydrogen peroxide. SC has been used as the primary ethanol producer in industries because this microbe has a high tolerance for ethanol production. This property makes SC ideal for the production of ethanol in this process. The presence of BS does not directly play a part in ethanol production, but this microbe is used owing to its property of increased alcohol production from cellulose which is sugarcane bagasse in this case. The pretreatment conditions of alkaline hydrogen peroxide (H2O2) were optimized to the values 3 h, 50 °C, 60 psi, pH 8.6, and 150 rpm. The analysis indicates improved production through batch fermentation pretreatment at various operational stages. After pretreatment, different qualitative characterization techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), and Thermogravimetric Analysis (TGA) results confirmed the better effects of structural changes of hemicellulose, lignin, and cellulose, mass loss, thermal degradation, and higher concentration of bioethanol in the distillate. The conversion of biomass to bioethanol using SC yields a high production yield (70%), with a production of 70 g/L from 100 g of SCB after 72 h and a bioethanol yield (0.7 g/g) of SCB confirmed through quantitative analysis from gas chromatography/mass spectrometry (GC/MS) and High-Performance Liquid Chromatography (HPLC).

Graphical Abstract