Scholarlink

<p>Mangosteen pericarps, a rich source of bioactive xanthones, particularly α-mangostin and γ-mangostin, were the focus of this study. The primary objective was to design and experimentally implement a continuous separation process using a three-zone simulated moving bed (TZ-SMB) system to isolate and purify these valuable compounds. Xanthone powder, extracted from mangosteen pericarps using acetonitrile and purified by anti-solvent precipitation, served as feedstock. This powder, with an initial α-mangostin purity of 71.56%, was subjected to separation on a single C18 preparative column to determine crucial adsorption parameters, including linear adsorption isotherms and mass transfer coefficients. A mobile phase consisting of 75% v/v acetonitrile was found to effectively separate α-mangostin and γ-mangostin. Subsequently, computational simulations based on triangle theory were employed to optimize TZ-SMB operating parameters. The optimal conditions involved a 20-min switching time and flow rates of 5.00, 1.062, 2.425, and 3.637&#xa0;mL/min for the mobile phase, feed, extract, and raffinate, respectively. Under these conditions, the system achieved a maximum productivity of 0.56&#xa0;mg/mL·h while maintaining high purities for both α-mangostin and γ-mangostin in the respective products. Experimental validation of the TZ-SMB system, using slightly adjusted flow rates, resulted in an α-mangostin purity of 100% in the extract product and a γ-mangostin purity of 98.79% in the raffinate product. The dried extract product exhibited an α-mangostin purity of 99.4% (HPLC grade). This research highlights the potential of TZ-SMB as a promising technology for the efficient and scalable purification of bioactive compounds from natural sources.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Continuous Separation of Alpha-Mangostin and Gamma-Mangostin Fractions from Xanthone Extracted from Mangosteen Pericarps Using a Preparative Three-Zone Simulated Moving Bed System

  • 期刊论文

  • 发表日期：

    2025年7月3日

查看全文
  • Preuk Tangpromphan,
  • Amaraporn Kaewchada,
  • Attasak Jaree

摘要

Mangosteen pericarps, a rich source of bioactive xanthones, particularly α-mangostin and γ-mangostin, were the focus of this study. The primary objective was to design and experimentally implement a continuous separation process using a three-zone simulated moving bed (TZ-SMB) system to isolate and purify these valuable compounds. Xanthone powder, extracted from mangosteen pericarps using acetonitrile and purified by anti-solvent precipitation, served as feedstock. This powder, with an initial α-mangostin purity of 71.56%, was subjected to separation on a single C18 preparative column to determine crucial adsorption parameters, including linear adsorption isotherms and mass transfer coefficients. A mobile phase consisting of 75% v/v acetonitrile was found to effectively separate α-mangostin and γ-mangostin. Subsequently, computational simulations based on triangle theory were employed to optimize TZ-SMB operating parameters. The optimal conditions involved a 20-min switching time and flow rates of 5.00, 1.062, 2.425, and 3.637 mL/min for the mobile phase, feed, extract, and raffinate, respectively. Under these conditions, the system achieved a maximum productivity of 0.56 mg/mL·h while maintaining high purities for both α-mangostin and γ-mangostin in the respective products. Experimental validation of the TZ-SMB system, using slightly adjusted flow rates, resulted in an α-mangostin purity of 100% in the extract product and a γ-mangostin purity of 98.79% in the raffinate product. The dried extract product exhibited an α-mangostin purity of 99.4% (HPLC grade). This research highlights the potential of TZ-SMB as a promising technology for the efficient and scalable purification of bioactive compounds from natural sources.

Graphical Abstract