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Purpose <p>This study aims to evaluate the bioactive potential of dry coffee parchment powder (DCPP) by investigating its phytochemical composition specifically polyphenols, flavonoids, and condensed tannins as well as its in vitro biological properties and in vivo acute toxicity effects.</p> Methods <p>The research involved phytochemical quantification of aqueous coffee parchment extract (ACPE) to determine polyphenol, flavonoid, and condensed tannin content. The biological properties were assessed through antioxidant activity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate cell viability at different ACPE concentrations (375–1500&#xa0;µg/ml). Additionally, in vivo acute toxicity tests were conducted on male and female rats at varying DCPP doses (25%, 50%, 75%, and 85%).</p> Results <p>The ACPE was found to be rich in polyphenols (105.793 ± 4.230&#xa0;mg GAE/g DW), flavonoids (13.147 ± 4.169&#xa0;mg CE/g DW), and condensed tannins (82.07 ± 3.942&#xa0;mg CE/g DW). The extract exhibited more than moderate antioxidant potency. The MTT assay revealed that cell viability remained above 50% at lower ACPE concentrations. Acute toxicity tests indicated that high doses of DCPP (75% and 85%) led to physiological changes, with females being more affected, highlighting a sex-based difference in response.</p> Conclusion <p>Dry coffee parchment powder presents promising bioactive properties and can be used as a natural additive in controlled doses across various applications, including livestock feed, cosmetics, and pharmaceuticals.</p>

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Dry Coffee Parchment Powder as a Source of Polyphenolic Compounds: A Comprehensive Study on its Phytochemistry, Bioactivities, and Toxicity Insights

  • 期刊论文

  • 发表日期：

    2025年6月11日

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  • Nesrine Fatima Zohra Benoussar,
  • Nahida Haddam,
  • Nabila Belyagoubi-Benhammou,
  • Fatima Zahra Tahir,
  • Yamna Kherraf,
  • Salim Habi,
  • Fatiha Benmeliani,
  • Asma Benchouk Bestaoui,
  • Erdal Eroglu,
  • Hafida Merzouk

摘要

Purpose

This study aims to evaluate the bioactive potential of dry coffee parchment powder (DCPP) by investigating its phytochemical composition specifically polyphenols, flavonoids, and condensed tannins as well as its in vitro biological properties and in vivo acute toxicity effects.

Methods

The research involved phytochemical quantification of aqueous coffee parchment extract (ACPE) to determine polyphenol, flavonoid, and condensed tannin content. The biological properties were assessed through antioxidant activity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate cell viability at different ACPE concentrations (375–1500 µg/ml). Additionally, in vivo acute toxicity tests were conducted on male and female rats at varying DCPP doses (25%, 50%, 75%, and 85%).

Results

The ACPE was found to be rich in polyphenols (105.793 ± 4.230 mg GAE/g DW), flavonoids (13.147 ± 4.169 mg CE/g DW), and condensed tannins (82.07 ± 3.942 mg CE/g DW). The extract exhibited more than moderate antioxidant potency. The MTT assay revealed that cell viability remained above 50% at lower ACPE concentrations. Acute toxicity tests indicated that high doses of DCPP (75% and 85%) led to physiological changes, with females being more affected, highlighting a sex-based difference in response.

Conclusion

Dry coffee parchment powder presents promising bioactive properties and can be used as a natural additive in controlled doses across various applications, including livestock feed, cosmetics, and pharmaceuticals.