Scholarlink

<p>Pulsed Electric Field (PEF) assisted acid hydrolysis was explored as an efficient approach to convert damaged pearl millet (DPM) into fermentable sugars for polyhydroxybutyrate (PHB) production. Designed experiment using response surface methodology was employed to optimize the processing parameters responsible for PEF-assisted acid hydrolysis, including solid-to-solvent ratio, PEF treatment time, and electric field strength having three levels of each. The optimal conditions were identified as a solid-to-solvent ratio of 1:4 (w/v), PEF treatment time of 30&#xa0;s, and electric field strength of 20&#xa0;kV/cm, resulting maximum reducing sugar concentration of 69.9&#xa0;g/L. Further, fermentation using <i>Bacillus subtilis</i> was carried out in which hydrolysate obtained from PEF-assisted DPM hydrolysis served as a carbon source, resulting in a maximum PHB concentration of 1.47&#xa0;g/L, corresponding to 50% of the dry cell biomass at 48&#xa0;h of incubation period. Pearl millet hydrolysate accumulated more PHB than glucose (control), highlighting its potential as a sustainable feedstock. Nile Red staining confirmed intracellular PHB accumulation, corroborating the gravimetric analysis. The study reveals that PEF-assisted hydrolysis can enhance sugar recovery if agricultural waste like damaged pearl millet is used as a substrate, enabling sustainable PHB production. Damaged pearl millet hydrolysate can serve as a viable feedstock for PHB synthesis, contributing to circular economy practices by transforming agricultural waste into value-added bioplastics.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Polyhydroxybutyrate Production by Bacillus subtilis Using Pulsed Electric Field-Assisted Acid Hydrolysate of Damaged Pearl Millet

  • 期刊论文

  • 发表日期：

    2025年5月27日

查看全文
  • Purnima Kumari,
  • Anupama Singh

摘要

Pulsed Electric Field (PEF) assisted acid hydrolysis was explored as an efficient approach to convert damaged pearl millet (DPM) into fermentable sugars for polyhydroxybutyrate (PHB) production. Designed experiment using response surface methodology was employed to optimize the processing parameters responsible for PEF-assisted acid hydrolysis, including solid-to-solvent ratio, PEF treatment time, and electric field strength having three levels of each. The optimal conditions were identified as a solid-to-solvent ratio of 1:4 (w/v), PEF treatment time of 30 s, and electric field strength of 20 kV/cm, resulting maximum reducing sugar concentration of 69.9 g/L. Further, fermentation using Bacillus subtilis was carried out in which hydrolysate obtained from PEF-assisted DPM hydrolysis served as a carbon source, resulting in a maximum PHB concentration of 1.47 g/L, corresponding to 50% of the dry cell biomass at 48 h of incubation period. Pearl millet hydrolysate accumulated more PHB than glucose (control), highlighting its potential as a sustainable feedstock. Nile Red staining confirmed intracellular PHB accumulation, corroborating the gravimetric analysis. The study reveals that PEF-assisted hydrolysis can enhance sugar recovery if agricultural waste like damaged pearl millet is used as a substrate, enabling sustainable PHB production. Damaged pearl millet hydrolysate can serve as a viable feedstock for PHB synthesis, contributing to circular economy practices by transforming agricultural waste into value-added bioplastics.

Graphical Abstract