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<p>The improper storage and recalcitrant characteristics of lignocellulosic biomass severely limit its biogas production. To address this, we investigated the bioaugmented effects of additives (<i>Pichia anomala</i>, cellulase, or their combination) on ensiling pretreatment and the methanogenic potential of sweet sorghum. The experimental groups included untreated fresh sweet sorghum (SS), silages without additive (CK), <i>P. anomala</i>-inoculated (Pa), cellulase-supplemented (Cx), and their combination (PC). Both Pa and Cx, individually or in combination, enhanced the ensiling fermentation by increasing lactic and AA contents, accelerating the biomethane production rate, and shortening the lag phase during anaerobic digestion. Furthermore, the optimal bioaugmented ensiling was achieved by combining <i>P. anomala</i> and cellulase (PC) additives, followed by <i>P. anomala</i> alone (Pa). Notably, the maximum cumulative methane production reached 457.70&#xa0;mL CH<sub>4</sub>/g VS in PC silages, representing a 30.13% increase over the SS control. Correspondingly, PC silages showed a maximum biodegradation rate of 72.39%, and the lag phase decreased by 62.96% compared to SS. In essence, the ensiling pretreatment significantly enhanced microbial taxa (e.g., <i>Firmicutes</i> and <i>Bacteroidota</i>), which are recognized for their lignocellulose-degrading functions and may synergistically increase bacteria and archaea diversity in the AD system. In short, combining cellulase and <i>P. anomala</i> is a potential option to enhance ensiling preservation and improve the biomethane production potential of sweet sorghum.</p>

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Enhancement of Bio-Conservation and Bio-Methane Production of Sweet Sorghum Silage by the addition of Pichia anomala and Cellulase

  • 期刊论文

  • 发表日期：

    2025年6月5日

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  • Jinju Liu,
  • Zhongqi Li,
  • Haiwei Ren,
  • Wenhao Ding

摘要

The improper storage and recalcitrant characteristics of lignocellulosic biomass severely limit its biogas production. To address this, we investigated the bioaugmented effects of additives (Pichia anomala, cellulase, or their combination) on ensiling pretreatment and the methanogenic potential of sweet sorghum. The experimental groups included untreated fresh sweet sorghum (SS), silages without additive (CK), P. anomala-inoculated (Pa), cellulase-supplemented (Cx), and their combination (PC). Both Pa and Cx, individually or in combination, enhanced the ensiling fermentation by increasing lactic and AA contents, accelerating the biomethane production rate, and shortening the lag phase during anaerobic digestion. Furthermore, the optimal bioaugmented ensiling was achieved by combining P. anomala and cellulase (PC) additives, followed by P. anomala alone (Pa). Notably, the maximum cumulative methane production reached 457.70 mL CH4/g VS in PC silages, representing a 30.13% increase over the SS control. Correspondingly, PC silages showed a maximum biodegradation rate of 72.39%, and the lag phase decreased by 62.96% compared to SS. In essence, the ensiling pretreatment significantly enhanced microbial taxa (e.g., Firmicutes and Bacteroidota), which are recognized for their lignocellulose-degrading functions and may synergistically increase bacteria and archaea diversity in the AD system. In short, combining cellulase and P. anomala is a potential option to enhance ensiling preservation and improve the biomethane production potential of sweet sorghum.