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<p>Three unrecyclable waste-paper streams, namely label backing paper (LB), multilayer paper-based packaging (ML) and potato paper sacks (PS) were characterised as fermentation feedstocks. Industrial <i>Saccharomyces cerevisiae</i> strain Ethanol Red® and recombinant <i>S. cerevisiae</i> strains Cellusec®&#xa0;2.0 and Cellusec®&#xa0;3.3 engineered for both cellulase-secretion and xylose utilisation were compared. Fermentation performance using Ethanol Red® with batch simultaneous saccharification and fermentation (SSF) was higher compared to separate hydrolysis and fermentation (SHF), and pre-saccharification and SSF (PSSF). Shake flask fed-batch SSF at 30% (w/w) final solid loading produced the highest ethanol titres of 75.01, 49.9 and 82.81&#xa0;g/L for untreated LB, ML and PS, respectively, and yields above 70% of the theoretical maximum, at an&#xa0;enzyme dosage of 10 FPU/g dry solids (ds), using Ethanol Red<b>®</b>. However, Cellusec®&#xa0;2.0 and 3.3 in batch SSF at a two-to-three-fold reduced exogenous enzyme requirement of 3.5 FPU/g ds, achieved ethanol titres and yields higher than Ethanol Red<b>®</b>.</p> Graphical Abstract <p></p>

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Valorisation of Waste-Paper Streams to Ethanol Through Submerged Fermentation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月14日

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  • Juliana E. Naudé,
  • Daneal C. S. Rorke,
  • Johann F. Görgens,
  • Eugéne van Rensburg

摘要

Three unrecyclable waste-paper streams, namely label backing paper (LB), multilayer paper-based packaging (ML) and potato paper sacks (PS) were characterised as fermentation feedstocks. Industrial Saccharomyces cerevisiae strain Ethanol Red® and recombinant S. cerevisiae strains Cellusec® 2.0 and Cellusec® 3.3 engineered for both cellulase-secretion and xylose utilisation were compared. Fermentation performance using Ethanol Red® with batch simultaneous saccharification and fermentation (SSF) was higher compared to separate hydrolysis and fermentation (SHF), and pre-saccharification and SSF (PSSF). Shake flask fed-batch SSF at 30% (w/w) final solid loading produced the highest ethanol titres of 75.01, 49.9 and 82.81 g/L for untreated LB, ML and PS, respectively, and yields above 70% of the theoretical maximum, at an enzyme dosage of 10 FPU/g dry solids (ds), using Ethanol Red®. However, Cellusec® 2.0 and 3.3 in batch SSF at a two-to-three-fold reduced exogenous enzyme requirement of 3.5 FPU/g ds, achieved ethanol titres and yields higher than Ethanol Red®.

Graphical Abstract