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<p>Pacu (<i>Piaractus mesopotamicus</i>) is a widely cultivated freshwater fish, generating substantial solid waste during processing. This study aimed to characterize the alkaline proteases in pacu processing waste using biochemical techniques, providing insights into their digestive enzymes for future biotechnological applications. Viscera (<i>n</i> = 10) were divided into pyloric ceca (PC), anterior intestine (AI), and posterior intestine (PI) for enzymatic extraction. Alkaline protease specific activity (SA) was measured under various reaction conditions. PC showed the highest proteolytic activity (SA = 1.3 ± 0.17 U/mg protein), followed by AI (SA = 0.5 ± 0.13 U/mg protein). PI exhibited lower activity, suggesting it is unsuitable for protease extraction. PC and AI enzymes were stable across a pH range of 7 to 11, with optimal activity at pH 9 to 11. The optimum temperature for both organs was 55&#xa0;°C. Proteases were stable at 10&#xa0;°C and 25&#xa0;°C, with variable activity at 40&#xa0;°C and 55&#xa0;°C. CaCl₂ reduced activity, while NaCl had no effect. Tween-80 decreased activity significantly, whereas SDS and Triton had minimal impact. Pacu proteases have the potential for various industrial uses, adding value to fish farming waste and promoting sustainability in aquaculture.</p>

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Valorization of Pacu Aquaculture Processing Waste: Preliminary Characterization of Digestive Alkaline Proteases

  • 期刊论文

  • 发表日期：

    2025年3月22日

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  • Yamila Eliana Rodriguez,
  • María Victoria Laitano,
  • Clara Liebana,
  • Ivana Soledad Friedman,
  • Hernán Javier Sacristán,
  • Juana Cristina del Valle,
  • Analia Verónica Fernández-Gimenez

摘要

Pacu (Piaractus mesopotamicus) is a widely cultivated freshwater fish, generating substantial solid waste during processing. This study aimed to characterize the alkaline proteases in pacu processing waste using biochemical techniques, providing insights into their digestive enzymes for future biotechnological applications. Viscera (n = 10) were divided into pyloric ceca (PC), anterior intestine (AI), and posterior intestine (PI) for enzymatic extraction. Alkaline protease specific activity (SA) was measured under various reaction conditions. PC showed the highest proteolytic activity (SA = 1.3 ± 0.17 U/mg protein), followed by AI (SA = 0.5 ± 0.13 U/mg protein). PI exhibited lower activity, suggesting it is unsuitable for protease extraction. PC and AI enzymes were stable across a pH range of 7 to 11, with optimal activity at pH 9 to 11. The optimum temperature for both organs was 55 °C. Proteases were stable at 10 °C and 25 °C, with variable activity at 40 °C and 55 °C. CaCl₂ reduced activity, while NaCl had no effect. Tween-80 decreased activity significantly, whereas SDS and Triton had minimal impact. Pacu proteases have the potential for various industrial uses, adding value to fish farming waste and promoting sustainability in aquaculture.