<p>Biomass pretreatment is a crucial step in producing renewable energy products to meet the growing demand for biofuels, offering a promising avenue toward a sustainable future. Various biomasses are used to create solid, liquid, and gaseous fuels converted into heat or electricity to reduce the increasing demand for conventional fuels and rein in the perilous greenhouse gas emissions. Pretreatment technologies play a critical role in producing precursor molecules from biomass, affecting the production and quality of biofuels. Several pretreatment methods, such as physical, chemical, and biological, are used, but they are only somewhat efficient and often result in low yields. HOWEVER, combining these strategies, such as physicochemical, chemico-biological, and physico-biological, and their concatenation with advanced nanotechnology can provide a better solution. Advanced nanotechnology, in the case of biomass valorization, refers to the management of materials at the nanoscale to enhance efficiency and yield and reduce environmental impact. Furthermore, the review encompasses a comprehensive analysis of various biomass resources used for biofuel production, the significance of advancements integrating biomass pretreatment technologies, and techno-economic analysis as a critical enabler for advancing towards a more sustainable and resource-efficient energy paradigm.</p> Graphical Abstract <p></p>