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Purpose <p>Bioactive peptides derived from fish waste have attracted significant research interest in recent years because of their wide-ranging benefits, such as antioxidant, antihypertensive, and antimicrobial properties. This research aimed to generate dual-function peptides (ACE-inhibitory and antioxidant) from gelatine extracted from the bones of hybrid grouper via Alcalase hydrolysis. The antihypertensive and antioxidant effects are the most extensively investigated, with both exhibiting synergistic impact on cardiovascular health.</p> Methods <p>The Alcalase-treated bone gelatine hydrolysate was subjected to ultrafiltration. The fractions were analysed for ACE-inhibitory and antioxidant activities. The peptide sequences of the potent fraction were identified and subjected to in vitro gastrointestinal digestion.</p> Results <p>The Alcalase-treated bone gelatine hydrolysate demonstrated stronger ACE-inhibitory and antioxidant capacities than did the unhydrolysed bone gelatine. Fractionation of bone gelatine hydrolysate via ultrafiltration yielded four fractions of varying molecular weights. The fraction containing peptides less than 1&#xa0;kDa in size (ABGH-IV) presented the highest ACE-inhibitory and ferrous chelating activities (P &lt; 0.05), whereas the fraction with peptides greater than 5&#xa0;kDa in size (ABGH-I) presented greater hydroxyl radical scavenging and reducing power (P &lt; 0.05). Notably, ABGH-IV demonstrated a greater ACE-inhibitory effect than PeptACE (an established commercial antihypertensive supplement). Seventy-five novel ACE-inhibitory and antioxidant peptides were identified in ABGH-IV, 11 of which were predicted to be highly active based on PeptideRanker. These newly discovered peptides demonstrated stability against in vitro gastrointestinal digestion and resistance to ACE breakdown, suggesting their potential as natural ACE-inhibitor and antioxidants.</p> Conclusion <p>This study shows the potential of novel peptides isolated from hybrid grouper bone gelatine act against hypertension and oxidative stress.</p> Graphical abstract <p></p>

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Valorisation of Hybrid Grouper Bone Gelatine: Identification and In Silico Analysis of Novel ACE-Inhibitory and Antioxidant Peptides Obtained Via Ultrafiltration

  • 期刊论文

  • 发表日期：

    2025年2月27日

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  • Pei-Teng Chan,
  • Patricia Matanjun,
  • Chayo Budiman,
  • Rossita Shapawi,
  • Jau-Shya Lee

摘要

Purpose

Bioactive peptides derived from fish waste have attracted significant research interest in recent years because of their wide-ranging benefits, such as antioxidant, antihypertensive, and antimicrobial properties. This research aimed to generate dual-function peptides (ACE-inhibitory and antioxidant) from gelatine extracted from the bones of hybrid grouper via Alcalase hydrolysis. The antihypertensive and antioxidant effects are the most extensively investigated, with both exhibiting synergistic impact on cardiovascular health.

Methods

The Alcalase-treated bone gelatine hydrolysate was subjected to ultrafiltration. The fractions were analysed for ACE-inhibitory and antioxidant activities. The peptide sequences of the potent fraction were identified and subjected to in vitro gastrointestinal digestion.

Results

The Alcalase-treated bone gelatine hydrolysate demonstrated stronger ACE-inhibitory and antioxidant capacities than did the unhydrolysed bone gelatine. Fractionation of bone gelatine hydrolysate via ultrafiltration yielded four fractions of varying molecular weights. The fraction containing peptides less than 1 kDa in size (ABGH-IV) presented the highest ACE-inhibitory and ferrous chelating activities (P < 0.05), whereas the fraction with peptides greater than 5 kDa in size (ABGH-I) presented greater hydroxyl radical scavenging and reducing power (P < 0.05). Notably, ABGH-IV demonstrated a greater ACE-inhibitory effect than PeptACE (an established commercial antihypertensive supplement). Seventy-five novel ACE-inhibitory and antioxidant peptides were identified in ABGH-IV, 11 of which were predicted to be highly active based on PeptideRanker. These newly discovered peptides demonstrated stability against in vitro gastrointestinal digestion and resistance to ACE breakdown, suggesting their potential as natural ACE-inhibitor and antioxidants.

Conclusion

This study shows the potential of novel peptides isolated from hybrid grouper bone gelatine act against hypertension and oxidative stress.

Graphical abstract