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<p>The rich biological heritage of Brazil, especially that of the Amazon, stands out as a strategic environment for exploitation of new microorganisms. In this study, the endophytic fungus <i>Penicillium paxilli</i>, isolated from <i>Bertholletia excelsa</i> nuts, was applied to biotransformation of progesterone. Whole cells of this fungus were immobilized on silk fibroin (from the cocoon of <i>Bombyx mori</i>) and on chitin (from the shell of the Amazon shrimp). In all cases, testololactone was identified as the major product of the reaction: 92% in free cells, 75% in cells immobilized on silk fibroin and 54% in cells immobilized on chitin at 5&#xa0;days. After one month of biocatalyst storage, the system utilizing cells immobilized on silk fibroin demonstrated superior performance in maintaining the biocatalytic activity of the fungus <i>P. paxilli</i> for the biotransformation of progesterone into testololactone, compared to both free-cell systems and cells immobilized on chitin.</p>

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Biotransformation of Progesterone by Penicillium paxilli Isolated from Bertholletia excelsa Nuts: Immobilization on Silk Fibroin and Chitin

  • 期刊论文

  • 发表日期：

    2025年4月7日

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  • Janaína Araújo E. de Dourado,
  • Samuel Q. Lopes,
  • Marcos Accioly Jr,
  • Cristiano Raminelli,
  • David E. Q. Jimenez,
  • André L. M. Porto,
  • Irlon M. Ferreira

摘要

The rich biological heritage of Brazil, especially that of the Amazon, stands out as a strategic environment for exploitation of new microorganisms. In this study, the endophytic fungus Penicillium paxilli, isolated from Bertholletia excelsa nuts, was applied to biotransformation of progesterone. Whole cells of this fungus were immobilized on silk fibroin (from the cocoon of Bombyx mori) and on chitin (from the shell of the Amazon shrimp). In all cases, testololactone was identified as the major product of the reaction: 92% in free cells, 75% in cells immobilized on silk fibroin and 54% in cells immobilized on chitin at 5 days. After one month of biocatalyst storage, the system utilizing cells immobilized on silk fibroin demonstrated superior performance in maintaining the biocatalytic activity of the fungus P. paxilli for the biotransformation of progesterone into testololactone, compared to both free-cell systems and cells immobilized on chitin.