Scholarlink

<p>The fatty acids have been produced from vegetable oils and animal fats, worldwide. However, due to limited availability and high cost, this sector thus requires expansion in feedstock to more abundant lignocellulosic biomass. This study focuses on production of fatty acids from lignocellulosic biomass, specifically Sugarcane bagasse (SCB) and Sugarcane trash (SCT), after enzymatic hydrolysis and fermentation by engineered yeast <i>Pichia pastoris</i>. The NaOH catalyzed methanol pretreatment parameters for SCB and SCT were optimized using Box–Behnken design that resulted efficient delignification and its conversion into reducing sugars (6.8&#xa0;g/L). The enzymes cellulase from <i>Bacillus paralicheniformis</i> and xylanase from <i>Bacillus altitudinis</i> were statistically optimized with maximum specific activity up to 92.02 and 80.27 U/mg, respectively. Several different crude enzymatic cocktails and sequential approaches were adopted for saccharification of pretreated SCB and SCT. Sequential saccharification of pretreated biomass yielded total reducing sugars (TRS) 28.52&#xa0;g/L, containing 21.33&#xa0;g/L glucose and 7.18&#xa0;g/L xylose, indicates a fourfold (300%) high xylose yield in comparison to enzyme cocktails. This hydrolysate was efficiently co-utilized in the presence of 10&#xa0;g/L methanol by metabolically engineered <i>P. pastoris</i> K2161H producing 1292.71&#xa0;mg/L of free fatty acids (FFA). Glucose was completely consumed within 48&#xa0;h, followed by simultaneous utilization of methanol and xylose at 96th hour. This optimized process not only enhanced FFA yield but also offers a sustainable and cost-effective alternative to traditional methods. A combination of saturated, monounsaturated, and polyunsaturated FFAs were produced that could be advantageous for various industrial applications.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Production of Free Fatty Acids from Alkaline Organosolvent Pretreated Sugarcane Bagasse and Trash by Yeast Pichia pastoris

  • 期刊论文

  • 发表日期：

    2025年2月18日

查看全文
  • Salah Ud Din,
  • Ayesha Israr,
  • Sadia Jabeen,
  • Qurrat ul Ain Rana,
  • Salah Ud Din,
  • Zhang Kun,
  • Samiullah Khan,
  • Fariha Hasan,
  • Malik Badshah,
  • Yongjin J. Zhou,
  • Aamer Ali Shah

摘要

The fatty acids have been produced from vegetable oils and animal fats, worldwide. However, due to limited availability and high cost, this sector thus requires expansion in feedstock to more abundant lignocellulosic biomass. This study focuses on production of fatty acids from lignocellulosic biomass, specifically Sugarcane bagasse (SCB) and Sugarcane trash (SCT), after enzymatic hydrolysis and fermentation by engineered yeast Pichia pastoris. The NaOH catalyzed methanol pretreatment parameters for SCB and SCT were optimized using Box–Behnken design that resulted efficient delignification and its conversion into reducing sugars (6.8 g/L). The enzymes cellulase from Bacillus paralicheniformis and xylanase from Bacillus altitudinis were statistically optimized with maximum specific activity up to 92.02 and 80.27 U/mg, respectively. Several different crude enzymatic cocktails and sequential approaches were adopted for saccharification of pretreated SCB and SCT. Sequential saccharification of pretreated biomass yielded total reducing sugars (TRS) 28.52 g/L, containing 21.33 g/L glucose and 7.18 g/L xylose, indicates a fourfold (300%) high xylose yield in comparison to enzyme cocktails. This hydrolysate was efficiently co-utilized in the presence of 10 g/L methanol by metabolically engineered P. pastoris K2161H producing 1292.71 mg/L of free fatty acids (FFA). Glucose was completely consumed within 48 h, followed by simultaneous utilization of methanol and xylose at 96th hour. This optimized process not only enhanced FFA yield but also offers a sustainable and cost-effective alternative to traditional methods. A combination of saturated, monounsaturated, and polyunsaturated FFAs were produced that could be advantageous for various industrial applications.

Graphical Abstract