Scholarlink

<p>Anaerobic digestion is an effective method for producing biogas while simultaneously treating waste, yet challenges like acid and ammonia inhibition, often arise. Bioaugmentation offers a solution to these issues. In this study, <i>Bacillus subtilis</i> was introduced to modify the microbial community structure in a two-stage anaerobic digestion system utilizing chicken manure, corn straw, and food waste as substrates. The results demonstrated that a concentration of 2.24&#xa0;g/g VS of <i>Bacillus subtilis</i> yielded the highest outcomes, with cumulative hydrogen and methane production reaching 114.00 mL/g VS and 410.84 mL/g VS, respectively—representing increases of 35.88% and 37.54% compared to the control group. Furthermore, this concentration resulted in the highest methane proportion at 84.68%. Microbial analysis revealed that <i>Bacillus subtilis</i> significantly altered the microbial community, enhancing its relative abundance during the hydrogen production phase, which in turn elevated volatile fatty acids (VFAs) and hydrogen levels. In the methane production stage, <i>Bacillus subtilis</i> increased the relative abundance of key microbes such as Bacillus, Methanosarcina, Limnochordia, Syntrophomonas, Lentimicrobium, and Hydrogenispora, thus improving substrate decomposition efficiency and boosting methane yield. These findings suggest that <i>Bacillus subtilis</i> can effectively enhance both hydrogen and methane production in two-stage anaerobic digestion systems.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of Bacillus subtilis Bioaugmentation on Hydrogen-Methane Production and Microbial Community in a Two-Stage Anaerobic Digestion System

  • 期刊论文

  • 发表日期：

    2025年1月25日

查看全文
  • Xin Liu,
  • Xianpu Zhu,
  • Dominic Yellezuome,
  • Ronghou Liu,
  • Xuwei Liu,
  • Chen Sun,
  • Mohamed Hemida Abd-Alla,
  • Abdel-Hamied M. Rasmey

摘要

Anaerobic digestion is an effective method for producing biogas while simultaneously treating waste, yet challenges like acid and ammonia inhibition, often arise. Bioaugmentation offers a solution to these issues. In this study, Bacillus subtilis was introduced to modify the microbial community structure in a two-stage anaerobic digestion system utilizing chicken manure, corn straw, and food waste as substrates. The results demonstrated that a concentration of 2.24 g/g VS of Bacillus subtilis yielded the highest outcomes, with cumulative hydrogen and methane production reaching 114.00 mL/g VS and 410.84 mL/g VS, respectively—representing increases of 35.88% and 37.54% compared to the control group. Furthermore, this concentration resulted in the highest methane proportion at 84.68%. Microbial analysis revealed that Bacillus subtilis significantly altered the microbial community, enhancing its relative abundance during the hydrogen production phase, which in turn elevated volatile fatty acids (VFAs) and hydrogen levels. In the methane production stage, Bacillus subtilis increased the relative abundance of key microbes such as Bacillus, Methanosarcina, Limnochordia, Syntrophomonas, Lentimicrobium, and Hydrogenispora, thus improving substrate decomposition efficiency and boosting methane yield. These findings suggest that Bacillus subtilis can effectively enhance both hydrogen and methane production in two-stage anaerobic digestion systems.