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<p>Sugarcane bagasse was submitted to delignification using the laccase-enriched extract (specific activity of 0.45 U.mg<sup>−1</sup>) from <i>Xylaria</i> sp. isolated from cassava waste. The effect of temperature (21.3 to 46.7&#xa0;°C) and treatment time (0.2 to 5.8&#xa0;days) was evaluated using an experimental design on the lignin removal. The optimized condition was achieved at 21&#xa0;°C and two days, resulting in a delignification of 20.7%. The process efficiency was enhanced using different mediators (ABTS, syringaldazine, catechin, ferulic acid, and hydrolyzed lignin), achieving 31.2% lignin removal using syringaldazine and an increase in delignification efficiency of 51.1 ± 1.6% when adding the mediator compared to the results in its absence. Subsequently, the pretreated biomass was enzymatically hydrolyzed to get fermentable sugars. The glucose concentration achieved 33.0&#xa0;g.L<sup>−1</sup> and cellulose conversion of 43.6% after 48&#xa0;h, representing an increase of 56.0% in hydrolysis conversion compared with the control experiment (no laccase treatment) and a yield of 67.3% during the production of bioethanol from hydrolysate. Furthermore, no large amounts of wastewater were generated during the enzymatic pre-treatment process, showing a promising path for developing more sustainable biofuel production technologies.</p> Graphical Abstract <p></p>

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Optimization of Sugarcane Bagasse Pretreatment with Xylaria sp. Laccase for Bioethanol Production

  • 期刊论文

  • 发表日期：

    2025年2月4日

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  • Gabriel Rufino da Silva,
  • Bruna Pratto,
  • Andreia de Araújo Morandim-Giannetti

摘要

Sugarcane bagasse was submitted to delignification using the laccase-enriched extract (specific activity of 0.45 U.mg−1) from Xylaria sp. isolated from cassava waste. The effect of temperature (21.3 to 46.7 °C) and treatment time (0.2 to 5.8 days) was evaluated using an experimental design on the lignin removal. The optimized condition was achieved at 21 °C and two days, resulting in a delignification of 20.7%. The process efficiency was enhanced using different mediators (ABTS, syringaldazine, catechin, ferulic acid, and hydrolyzed lignin), achieving 31.2% lignin removal using syringaldazine and an increase in delignification efficiency of 51.1 ± 1.6% when adding the mediator compared to the results in its absence. Subsequently, the pretreated biomass was enzymatically hydrolyzed to get fermentable sugars. The glucose concentration achieved 33.0 g.L−1 and cellulose conversion of 43.6% after 48 h, representing an increase of 56.0% in hydrolysis conversion compared with the control experiment (no laccase treatment) and a yield of 67.3% during the production of bioethanol from hydrolysate. Furthermore, no large amounts of wastewater were generated during the enzymatic pre-treatment process, showing a promising path for developing more sustainable biofuel production technologies.

Graphical Abstract