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<p>With growing concerns over environmental pollution and sustainable energy sources, this study explores green coconut shell waste as a feedstock for bioethanol production. A two-step chemical pretreatment using NaClO and NaOH enhanced the accessibility of cellulose for enzymatic breakdown, resulting in high enzyme activity produced from <i>Bacillus pumilus</i> fermentation. The treated biomass underwent simultaneous saccharification and fermentation (SSF) with <i>Saccharomyces cerevisiae</i>, leading to a notable increase in bioethanol yield (0.514&#xa0;g/g) and productivity (0.177&#xa0;g/L/h), compared to untreated samples. Structural analyses, including FESEM and XRD, confirmed enhanced cellulose crystallinity and surface area post-treatment. These results demonstrate that optimized pretreatment significantly boosts bioethanol yield and presents an effective method for converting agricultural waste into biofuels. This study highlights the potential scalability of this approach for industrial bioethanol production, contributing to sustainable waste-to-energy solutions.</p>

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Bioethanol Production from Green Coconut Residue Using Xylanases and Cellulases in Simultaneous Saccharification and Fermentation

  • 期刊论文

  • 发表日期：

    2025年1月4日

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  • Shaina Sharma,
  • Simarpreet Kaur Gill,
  • Sudarshan Sahu,
  • Gursharan Singh,
  • Shailendra Kumar Arya,
  • Murugesan Chandrasekaran,
  • Mansour K. Gatasheh,
  • Sumathi Jones,
  • Balasubramani Ravindran,
  • Soon Woong Chang,
  • Ramesh Vediyappan

摘要

With growing concerns over environmental pollution and sustainable energy sources, this study explores green coconut shell waste as a feedstock for bioethanol production. A two-step chemical pretreatment using NaClO and NaOH enhanced the accessibility of cellulose for enzymatic breakdown, resulting in high enzyme activity produced from Bacillus pumilus fermentation. The treated biomass underwent simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae, leading to a notable increase in bioethanol yield (0.514 g/g) and productivity (0.177 g/L/h), compared to untreated samples. Structural analyses, including FESEM and XRD, confirmed enhanced cellulose crystallinity and surface area post-treatment. These results demonstrate that optimized pretreatment significantly boosts bioethanol yield and presents an effective method for converting agricultural waste into biofuels. This study highlights the potential scalability of this approach for industrial bioethanol production, contributing to sustainable waste-to-energy solutions.