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<p>This study investigates the partial purification, characterization, and application of lipase from <i>Yarrowia lipolytica</i> IMUFRJ50682, produced via solid-state fermentation (SSF), for omega-6 enrichment through fish oil hydrolysis. Lipase production utilized a fermentation substrate composed of soybean meal and andiroba cake (1:1) and was partially purified using an aqueous two-phase system (ATPS) formulated with 30% (wt) polyethylene glycol (PEG) 2000 and buffer salts (pH 7), containing 7% (wt) of potassium phosphate, citrate, or acetate. The effects of buffer salt type and additive concentration on ATPS performance for the partial purification of lipase and protease were systematically evaluated. The partially purified enzyme extract (PPEE) and crude enzyme extract (CEE) were characterized and applied to fish oil hydrolysis, targeting omega-6 enrichment. With phosphate buffer salts (pH 7), lipase recovery reached 98.92 ± 0.12–100%, with a purification factor of twofold. Protease preferentially migrated to the top phase, reducing undesired lipolytic degradation. PPEE exhibited peak activity at 30–45&#xa0;°C and pH 7–8, showing tolerance to ethanol, hexane, and acetone while remaining independent of Mg<sup>2</sup>⁺ and Na⁺ as cofactors. The omega-6 content in the hydrolyzed product increased fourfold with CEE and 12-fold with PPEE. This study highlights the efficient partial purification of lipase from <i>Y. lipolytica</i> using SSF and ATPS, demonstrating its potential for omega-6 enrichment from fish oil.</p> Graphical Abstract <p></p>

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Lipase Production Through Solid-State Fermentation: Partial Purification and Application for Omega-6 Enrichment in Fish Oil

  • 期刊论文

  • 发表日期：

    2025年1月25日

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  • Aparecida Selsiane Sousa Carvalho,
  • Filipe Smith Buarque,
  • Vanessa Naciuk Castelo Branco,
  • Célio Santos de Faria Júnior,
  • Rachel de Moraes Ferreira,
  • Bernardo Dias Ribeiro,
  • Ailton Cesar Lemes,
  • Maria Alice Zarur Coelho

摘要

This study investigates the partial purification, characterization, and application of lipase from Yarrowia lipolytica IMUFRJ50682, produced via solid-state fermentation (SSF), for omega-6 enrichment through fish oil hydrolysis. Lipase production utilized a fermentation substrate composed of soybean meal and andiroba cake (1:1) and was partially purified using an aqueous two-phase system (ATPS) formulated with 30% (wt) polyethylene glycol (PEG) 2000 and buffer salts (pH 7), containing 7% (wt) of potassium phosphate, citrate, or acetate. The effects of buffer salt type and additive concentration on ATPS performance for the partial purification of lipase and protease were systematically evaluated. The partially purified enzyme extract (PPEE) and crude enzyme extract (CEE) were characterized and applied to fish oil hydrolysis, targeting omega-6 enrichment. With phosphate buffer salts (pH 7), lipase recovery reached 98.92 ± 0.12–100%, with a purification factor of twofold. Protease preferentially migrated to the top phase, reducing undesired lipolytic degradation. PPEE exhibited peak activity at 30–45 °C and pH 7–8, showing tolerance to ethanol, hexane, and acetone while remaining independent of Mg2⁺ and Na⁺ as cofactors. The omega-6 content in the hydrolyzed product increased fourfold with CEE and 12-fold with PPEE. This study highlights the efficient partial purification of lipase from Y. lipolytica using SSF and ATPS, demonstrating its potential for omega-6 enrichment from fish oil.

Graphical Abstract