Synthesis and Characterization of Nitrogen-Doped Carbon Dots from Carissa carandoss for Environmental and In-vitro Biomedical Applications

The antibacterial impacts on human pathogens have been studied with aid of synthesized Niteogen doped Carbon Dots (N-CDs) originated from Carissa carandas. Extensive analysis of these N-CDs was conducted using a variety of techniques such as XRD, Raman, UV-Vis, Fluorescence spectrum, FT-IR, XPS, HR-TEM, and EDS, providing detailed insights into their structure, morphology, and surface characteristics. One of the key features of the N-CDs is their distinct green light emission at 478 nm, as established by fluorescence spectroscopy. Their unique spherical shape, with an average diameter of 5.64 ± 0.18 nm, was unveiled using high-resolution transmission electron microscopy (HRTEM). The successful incorporation of nitrogen into the carbon dots was verified through X-ray photoelectron spectroscopy (XPS) analysis. The study’s importance is further emphasized by the N-CDs’ effective antibacterial properties against various human pathogens such as Streptococcus Pyogenes, Staphylococcus Aureus, Klebsiella Pneumoniae, and Shigella Flexneri. Notably, the most effective antibacterial action was observed against Staphylococcus Aureus and Klebsiella Pneumoniae at a concentration of 100 µg/ml. In addition, these N-CDs demonstrated significant antioxidant and anti-inflammatory properties at the same concentration, as shown by the DPPH radical assay and HRBC cell line tests. This comprehensive evaluation of the N-CDs highlights their potential as a powerful and versatile antibacterial agent, offering promising new directions in combating bacterial infections.