Licuri (Syagrus coronata) Oil Cake as a Substrate for Collagenolytic Protease Production by Submerged Fermentation Using a Novel Strain of Penicillium citrinum Isolated from the Brazilian Caatinga

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发表日期：

2024年9月19日

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Diego Gomes Ramos,
Joenny Maria Silveira de Lima,
José Pedro Martins Barbosa Filho,
Cristina Maria de Souza-Motta,
Daniela de Araújo Viana Marques,
Marcia Vanusa da Silva,
Maria Tereza dos Santos Correia,
Romero Marcos Pedrosa Brandão Costa,
Attilio Converti,
Sinara Mônica Vitalino de Almeida,
Carolina de Albuquerque Lima Duarte

摘要

Purpose

This work aimed to produce, purify, and characterize a collagenase from a new strain of Penicillium citrinum isolated from Caatinga, introducing the residue from licuri (Syagrus coronata) oil extraction as a substrate.

Methods

Collagenase production through 72-h submerged fermentation was optimized by response surface methodology, varying the substrate concentration, temperature, initial pH of the medium, and orbital agitation, following a 2⁴-factorial design. The enzymatic purification used low and high-resolution processes, and the enzyme was characterized by their physicochemical properties.

Results

Our results showed that the maximum collagenolytic activity, obtained under optimized conditions, was 295.56 U/mL. Collagenase was stable at a wide range of pH (6–10) and temperature (25–40 °C), with optimal activity at 37 °C and pH 9. Enzyme activity was completely inhibited by phenylmethylsulfonyl fluoride (PMSF) and Zn⁺, while it was promoted by Na⁺ (by 43%), Ca²⁺ (by 8%), and β-mercaptoethanol (by 39%).

Conclusions

By introducing a natural residue as a promising substrate in addition to a new collagenase-producing strain, these results highlight the potential of resources from the Caatinga biome to produce new industrially useful enzymes.

Graphical Abstract