Environment-Friendly and Efficient Colorimetric Sensing of Hydrogen Peroxide Based on Dalbergia sissoo Sawdust-Deposited Nickel Nanoparticles

Hydrogen peroxide is a byproduct of respiration and an end product of various metabolic processes, such as a possible signal transmitter and peroxisomal oxidation pathways. It is one of the most common biological reactive oxygen species that can greatly affect tissues and cells. Monitoring the quantity of hydrogen peroxide with selectivity and peculiar sensitivity is required for the quantitative monitoring of hydrogen peroxide within the body. Herein, we developed a simple, environment-friendly, cost-effective, and quick approach for detecting hydrogen peroxide with high selectivity and sensitivity. Dalbergia sissoo sawdust based deposited nickel nanoparticles (Ni NPs) were synthesized using a standard hydrothermal process. The Formation of Ni NPs was analyzed by UV-Vis, FTIR, XRD, SEM, and EDX analysis techniques. As a sensor for hydrogen peroxide, the colorimetric change in TMB solution from colorless to blue-green was measured using UV-Vis spectroscopy. For optimal colorimetric response, different effecting parameters, including pH, NP loading effect, TMB concentration, and time were tuned. The proposed sensor worked best at pH 4, 2 mg NPs, 40 mM TMB, and a 5-minute incubation time. The desired sensor’s linear range is 20–110 µM with an R² value of 0.994. The suggested sensor’s limits of detection and quantification are 0.26 and 0.88 µM, respectively. Notably, these Ni NPs demonstrated good antimicrobial activities against the Salmonella typhii bacterium and the fungus Candida albicans.