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<p>Tissue samples collected from 163 slaughtered pigs were examined by conventional and molecular methods to determine the prevalence and characterization of <i>Sarcocystis suihominis</i> from south Indian pigs. Microscopy of tissue samples of each pig by intact cyst isolation and pepsin digestion method revealed sarcocysts in 3.7% and 12.3% of samples, respectively. Histopathological examination of the affected muscle tissues revealed mild leucocytic infiltration, myositis, hyaline and fatty degeneration surrounding the sarcocysts. The characteristics of tissue cysts were suggestive of <i>S. suihominis</i>, with a smooth and thin cyst wall. Genomic DNA was extracted individually from tissues of 163 pigs to amplify partial segment of 18&#xa0;S rRNA gene of <i>Sarcocystis</i> spp. Digestion of PCR amplicons (915&#xa0;bp) with restriction endonuclease revealed single electromorph which was referred to <i>S</i>. <i>suihominis</i> (915&#xa0;bp). The sequences from <i>S</i>. <i>suihominis</i> isolates showed 99.8 to100% identities with the Indian isolates of <i>S. suihominis</i> as a single monophyletic group along with Italian isolate of <i>S. suihominis</i> and formed a sister clade with <i>S</i>. <i>miescheriana</i>. Overall, the prevalence of <i>S. suihominis</i> was 20.9%. Age and breed influenced the prevalence of sarcocystosis being high in adults and non-descriptive pigs. The higher prevalence of <i>S. suihominis</i> in the study area incidentally specifies high rate of <i>S. suihominis</i> in human and a high degree of habitation contamination with human feces. Results highlight the need of public health education on avoiding eating raw or under cooked pork to prevent human infection.</p>

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Prevalence and characterization of zoonotic protozoa Sarcocystis suihominis from south Indian pigs

  • 期刊论文

  • 发表日期：

    2026年5月26日

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  • Madhuri Gatta,
  • JyothiSree Chitichoti ,
  • Sreedevi Chennuru,
  • Sudhakar Krovvidi

摘要

Tissue samples collected from 163 slaughtered pigs were examined by conventional and molecular methods to determine the prevalence and characterization of Sarcocystis suihominis from south Indian pigs. Microscopy of tissue samples of each pig by intact cyst isolation and pepsin digestion method revealed sarcocysts in 3.7% and 12.3% of samples, respectively. Histopathological examination of the affected muscle tissues revealed mild leucocytic infiltration, myositis, hyaline and fatty degeneration surrounding the sarcocysts. The characteristics of tissue cysts were suggestive of S. suihominis, with a smooth and thin cyst wall. Genomic DNA was extracted individually from tissues of 163 pigs to amplify partial segment of 18 S rRNA gene of Sarcocystis spp. Digestion of PCR amplicons (915 bp) with restriction endonuclease revealed single electromorph which was referred to S. suihominis (915 bp). The sequences from S. suihominis isolates showed 99.8 to100% identities with the Indian isolates of S. suihominis as a single monophyletic group along with Italian isolate of S. suihominis and formed a sister clade with S. miescheriana. Overall, the prevalence of S. suihominis was 20.9%. Age and breed influenced the prevalence of sarcocystosis being high in adults and non-descriptive pigs. The higher prevalence of S. suihominis in the study area incidentally specifies high rate of S. suihominis in human and a high degree of habitation contamination with human feces. Results highlight the need of public health education on avoiding eating raw or under cooked pork to prevent human infection.