<p>Helminth infection continues to adversely affect poultry health and productivity, especially in tropical and subtropical areas whereby resistance to traditional anthelmintic drugs is becoming common. The traditional medicinal plants can become a viable alternative because they are readily available, cheap and contain a variety of bioactive compounds. The indigenous communities of Assam use <i>Sarcochlamys pulcherrima</i> (Urticaceae) to treat various helminthic infections, but its anthelmintic effect is not scientifically verified. The present study evaluates the in vitro anthelmintic potential of the methanolic extract of <i>S. pulcherrima</i> leaves against <i>Raillietina echinobothrida</i>. Anthelmintic activity was evaluated in terms of motility index. The morphological changes were studied by scanning electron microscopy (SEM) and biochemical assays were performed to study the ATPase, AcPase and AlkPase activity. Our finding revealed that the plant extract (30&#xa0;mg/ml) caused rapid paralysis with total loss of motility in 6&#xa0;h, an earlier event compared to the standard drug. Major tegumental changes were noted with SEM, such as tegument erosion, wrinkling and folding and scolex and sucker deformities. Biochemical tests proved that there was a great inhibition of acid phosphatase (64%), alkaline phosphatase (59%) and ATPase (63%) activity in the extract-treated worms relative to the untreated control. The study provides scientific support for the traditional use of <i>S. pulcherrima</i> and highlights its potential as a natural source of anthelmintic agents. Further investigations, including bioassay-guided fractionation, toxicity evaluation and in vivo validation, are ongoing to establish <i>S. pulcherrima</i> as a safe and effective anthelmintic.</p>