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<p>Ticks are considered to be the important vectors of many disease-causing pathogens in animals and humans. For strategic control of pest or pathogens, their identification and epidemiological knowledge is very much essential. Accordingly, a total of 860 cattle were examined from four districts of western India where 46.05% (<i>n</i> = 396) cattle were found to be infested with ticks. The collected ticks were morphologically identified as either <i>Hyalomma anatolicum</i> or <i>Rhipicephalus microplus</i>. Which was further confirmed by PCR assay targeting cytochrome c oxidase subunit I (cox1) gene followed by sequence analysis. The interspecific divergence between current isolates of <i>H. anatolicum</i> and <i>R. microplus</i> was 20.9%. The wider range of intraspecific divergence was recorded in <i>R. microplus</i> (0–11.7%) compared to <i>H. anatolicum</i> (0–1.6%), globally. In phylogenetic analysis Indian isolates of <i>R. microplus</i> clustered with <i>R. microplus</i> clade C. Additionally, a more applicable test, In-silico followed by PCR-RFLP restriction enzyme analysis, was employed to differentiate between the two tick species. Among the total 396 tick infested cattle, significantly higher (<i>p</i> &lt; 0.001) number of cattle were found to be infested with <i>H. anatolicum</i> (70.96%, <i>n</i> = 281) as compared to <i>R. microplus</i> (51.77%, <i>n</i> = 205) whereas, 22.73% (<i>n</i> = 90) cattle were found to be infested with mixed tick infestation of both.</p>

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Morphological and molecular characterization of Ixodid ticks infesting cattle in western Gujarat, India

  • 期刊论文

  • 发表日期：

    2026年3月30日

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  • Jeemi Arbindbhai Patel,
  • Binod Kumar,
  • Bhupendrakumar Jamsubhai Thakre,
  • Biswa Ranjan Maharana,
  • Nilima Nayankumar Brahmbhatt,
  • Vivek Kumar Singh

摘要

Ticks are considered to be the important vectors of many disease-causing pathogens in animals and humans. For strategic control of pest or pathogens, their identification and epidemiological knowledge is very much essential. Accordingly, a total of 860 cattle were examined from four districts of western India where 46.05% (n = 396) cattle were found to be infested with ticks. The collected ticks were morphologically identified as either Hyalomma anatolicum or Rhipicephalus microplus. Which was further confirmed by PCR assay targeting cytochrome c oxidase subunit I (cox1) gene followed by sequence analysis. The interspecific divergence between current isolates of H. anatolicum and R. microplus was 20.9%. The wider range of intraspecific divergence was recorded in R. microplus (0–11.7%) compared to H. anatolicum (0–1.6%), globally. In phylogenetic analysis Indian isolates of R. microplus clustered with R. microplus clade C. Additionally, a more applicable test, In-silico followed by PCR-RFLP restriction enzyme analysis, was employed to differentiate between the two tick species. Among the total 396 tick infested cattle, significantly higher (p < 0.001) number of cattle were found to be infested with H. anatolicum (70.96%, n = 281) as compared to R. microplus (51.77%, n = 205) whereas, 22.73% (n = 90) cattle were found to be infested with mixed tick infestation of both.