<p><i>Toxoplasma gondii</i> is a widespread zoonotic parasite. This investigation aimed to determine the prevalence of <i>T. gondii</i> infection and associated risk factors in animals in the Kurdistan region of northeastern Iraq. Blood samples from 482 animals (224 sheep, 172 goats, 48 horses, and 38 donkeys) were collected randomly for serological and molecular examinations using the indirect fluorescent antibody test (IFAT) and nested PCR, respectively. The overall seroprevalence of <i>T. gondii</i> infection was 22.2% (95% CI: 18.5–25.9%), with rates of 33.3% in horses, 26.8% in sheep, 18.4% in donkeys, and 14% in goats. All seropositive samples were confirmed by molecular biology. The infection rate was 18.7% in males and 23.4% in females (<i>p</i> = 0.601); additionally, 26.7% of animals in close contact with cats were positive for <i>T. gondii</i> infection (<i>p</i> = 0.991). The infection rate in equids (26.7%) was higher than in small ruminants (21.2%); however, the difference was not significant (<i>p</i> = 0.607). In statistical evaluation, there were no significant differences (<i>p</i> &gt; 0.05) among infection rates, age of animals, sex of animals, and their contact with cats at the level of animal species. This work presents the first overview of animal toxoplasmosis in northeastern Iraq and provides a baseline for further investigations. Our findings suggest that toxoplasmosis may partially contribute to the transmission of <i>T. gondii</i> to humans and to economic losses in animals. Improving sanitary strategies to reduce risk factors in animal husbandry is recommended. Given the zoonotic nature of <i>T. gondii</i> and the relatively high seroprevalence in food animals, there is potential for transmission to humans through consumption of undercooked meat. Although we did not measure human infection in this study, our results support the continuation of public health advice to consume well-cooked meat.</p>