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<p>Coccidiosis is a serious disease in rabbits, can affect both the liver and intestines. Hepatic coccidiosis is caused by <i>Eimeria stiedae</i>, which inhabits the liver and bile ducts. In this study, 294 fecal samples were collected from local rabbits in Baghdad. Although, oocysts with distinctive morphology were found in the liver and bile ducts, they resembled those of intestinal species. To investigate this, experimental infections were conducted along with molecular identification techniques. Experimentally infected rabbits showed dilated bile ducts with developmental stages of <i>Eimeria</i> embedded in hyperplastic epithelium. PCR analysis of the <i>ITS1</i> region revealed that some isolates (Iq1–Iq3) had limited similarity with the known <i>E. stiedae</i>, while one (Iq4) was genetically closer to <i>E. irresidua.</i> These findings highlight the morphological overlap between hepatic and intestinal <i>Eimeria</i> species. The observed difference across isolates, may represent local species adaptability, host-parasite interactions, or even new species variety underline the need for molecular diagnosis to ensure accurate identification.</p>

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Gross and histopathological observations and genetic diversity of Eimeria species isolated from the liver and bile duct of experimentally infected rabbits in Iraq

  • 期刊论文

  • 发表日期：

    2025年10月8日

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  • Maulood M. Shather,
  • Dalia Ahmed Kalef,
  • Omar Hussein Khalaf,
  • Maab Salah Jameel

摘要

Coccidiosis is a serious disease in rabbits, can affect both the liver and intestines. Hepatic coccidiosis is caused by Eimeria stiedae, which inhabits the liver and bile ducts. In this study, 294 fecal samples were collected from local rabbits in Baghdad. Although, oocysts with distinctive morphology were found in the liver and bile ducts, they resembled those of intestinal species. To investigate this, experimental infections were conducted along with molecular identification techniques. Experimentally infected rabbits showed dilated bile ducts with developmental stages of Eimeria embedded in hyperplastic epithelium. PCR analysis of the ITS1 region revealed that some isolates (Iq1–Iq3) had limited similarity with the known E. stiedae, while one (Iq4) was genetically closer to E. irresidua. These findings highlight the morphological overlap between hepatic and intestinal Eimeria species. The observed difference across isolates, may represent local species adaptability, host-parasite interactions, or even new species variety underline the need for molecular diagnosis to ensure accurate identification.