Scholarlink

<p>Bioactive glasses (BGs) are renowned for their osteoconductive and regenerative properties, yet their clinical utility is limited by insufficient antibacterial performance. The growing concern over bacterial infections associated with open wound bone fractures and implant failures highlights the need for multifunctional biomaterials that combine regenerative and antibacterial capabilities. Several studies have demonstrated a significant strategy to enhance the antibacterial efficacy of BGs by incorporation of metal and metal oxide nanoparticles using chemically synthesized methods. However, chemical synthesis methods have limitations on biocompatibility. To overcome these limitations, in this study, a green synthesis approach was utilized to enhance both the antibacterial efficacy and biocompatibility of bioactive glass by incorporating silver and zinc oxide nanoparticles synthesized using an eco-friendly route using neem (Azadirachta indica) leaf extract. This green approach avoids the production of toxic by-products, while enhancing nanoparticle stability and biocompatibility. The structural and chemical identification, morphology, and specific surface areas of the synthesized BGs were characterized by XRD, FTIR, SEM, and BET analysis, respectively. Results confirmed the successful incorporation of Ag and zinc oxide (ZnO) without inducing crystallinity, maintaining the amorphous glass structure. The co-doped BG3 sample exhibited the highest pore volume and specific surface area, which resulted in enhanced <i>in vitro</i> bioactivity and biodegradability. For the MTT assay biocompatibility test, results revealed that all specimens exceeded the minimum required (70%) viability threshold. Antibacterial activity evaluation against Staphylococcus aureus and Escherichia coli via the disc diffusion method demonstrated that the co-doped sample at 2 wt% Ag and 2 wt% ZnO exhibited the highest antibacterial activity and <i>in vitro</i> bioactivity. Finally, the results confirmed that the Ag and ZnO co-doped BGs exhibited a significantly larger zone of inhibition compared to undoped samples, indicating strong antibacterial effects.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Green Synthesis of Silver and Zinc Oxide Nanoparticles Co-Doped Bioactive Glass for Enhancing Antibacterial Activity and In Vitro Biocompatibility

  • 期刊论文

  • 发表日期：

    2026年7月17日

查看全文
  • Yeabsira Sisay Mekuriya,
  • Tsion Chuni Akililu,
  • Kasim Sakran Abass,
  • Hailemariam Gebru,
  • Allah Dekama Jara,
  • Fetene Fufa Bakare

摘要

Bioactive glasses (BGs) are renowned for their osteoconductive and regenerative properties, yet their clinical utility is limited by insufficient antibacterial performance. The growing concern over bacterial infections associated with open wound bone fractures and implant failures highlights the need for multifunctional biomaterials that combine regenerative and antibacterial capabilities. Several studies have demonstrated a significant strategy to enhance the antibacterial efficacy of BGs by incorporation of metal and metal oxide nanoparticles using chemically synthesized methods. However, chemical synthesis methods have limitations on biocompatibility. To overcome these limitations, in this study, a green synthesis approach was utilized to enhance both the antibacterial efficacy and biocompatibility of bioactive glass by incorporating silver and zinc oxide nanoparticles synthesized using an eco-friendly route using neem (Azadirachta indica) leaf extract. This green approach avoids the production of toxic by-products, while enhancing nanoparticle stability and biocompatibility. The structural and chemical identification, morphology, and specific surface areas of the synthesized BGs were characterized by XRD, FTIR, SEM, and BET analysis, respectively. Results confirmed the successful incorporation of Ag and zinc oxide (ZnO) without inducing crystallinity, maintaining the amorphous glass structure. The co-doped BG3 sample exhibited the highest pore volume and specific surface area, which resulted in enhanced in vitro bioactivity and biodegradability. For the MTT assay biocompatibility test, results revealed that all specimens exceeded the minimum required (70%) viability threshold. Antibacterial activity evaluation against Staphylococcus aureus and Escherichia coli via the disc diffusion method demonstrated that the co-doped sample at 2 wt% Ag and 2 wt% ZnO exhibited the highest antibacterial activity and in vitro bioactivity. Finally, the results confirmed that the Ag and ZnO co-doped BGs exhibited a significantly larger zone of inhibition compared to undoped samples, indicating strong antibacterial effects.