<p>Ormosil, known for its unique attributes such as tuneable porosity, ability to possess a high amount of exposed surface area relative to their volume, and compatibility with living organisms, is promising as a biosensor material. This review covers current advances, methods, and obstacles associated with the use of Ormosil-based biosensors for the recognition of various analytes, including biomolecules, ions, and gases. Synthesis methods specifically tailored to biosensing are discussed, with an emphasis on strategies to brush up the sensitivity, selectivity, and stability of the sensors. It concludes by outlining prospects and challenges, emphasizing the potential of Ormosil-based biosensors in healthcare, environmental monitoring, industrial control processes, drug delivery and food safety. It highlights the need for interdisciplinary collaboration and innovative approaches to overcome current limitations and fully exploit Ormosil's capabilities in biosensor applications.</p> Graphical Abstract <p></p>