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<p><i>Lactiplantibacillus plantarum</i> stands out among probiotics for its strong environmental adaptability, diverse metabolic activity, proven safety, and broad functional benefits. To obtain safe <i>L. plantarum</i> strains with gut health–promoting properties, this study systematically screened numerous <i>L. plantarum strains</i> from human breast milk based on their acid and bile tolerance, hydrophobicity, autoaggregation, and antioxidant activity. Its cellular effects were assessed in Caco-2 and IEC-6 intestinal epithelial cell models by evaluating adhesion ability, cytotoxicity, cell cycle distribution, tight junction gene expression (e.g., ZO-1, Occludin), and LPS-induced inflammatory responses. Hemolytic activity and antibiotic resistance profiles were also assessed to ensure safety. Among the screened isolates, <i>Lactiplantibacillus plantarum</i> DPU-MWM14-103 (<i>L. plantarum</i> M14-103) demonstrated strong acid and bile resistance, high hydrophobicity, robust autoaggregation, and potent DPPH radical scavenging activity. Genome analysis revealed the absence of antibiotic resistance and virulence genes, along with the presence of genes associated with stress response and adhesion. In vitro assays confirmed its non-toxic nature, lack of hemolytic activity, and ability to enhance tight junction integrity and reduce inflammatory cytokine expression under LPS stimulation. As a result, <i>L. plantarum</i> M14-103 would be a potential probiotic strain for gut health.</p>

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Intestinal-Protective Lactiplantibacillus plantarum DPU-MWM14-103 Exhibits Genomic Adaptations and Multifaceted Probiotic Properties

  • 期刊论文

  • 发表日期：

    2026年6月1日

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  • Zhao Zhang,
  • Yanchen Liu,
  • Baoyuan Zhao,
  • Zhaobin Yan,
  • Xuemei Zhu,
  • Guangqing Mu,
  • Xiaomeng Wu

摘要

Lactiplantibacillus plantarum stands out among probiotics for its strong environmental adaptability, diverse metabolic activity, proven safety, and broad functional benefits. To obtain safe L. plantarum strains with gut health–promoting properties, this study systematically screened numerous L. plantarum strains from human breast milk based on their acid and bile tolerance, hydrophobicity, autoaggregation, and antioxidant activity. Its cellular effects were assessed in Caco-2 and IEC-6 intestinal epithelial cell models by evaluating adhesion ability, cytotoxicity, cell cycle distribution, tight junction gene expression (e.g., ZO-1, Occludin), and LPS-induced inflammatory responses. Hemolytic activity and antibiotic resistance profiles were also assessed to ensure safety. Among the screened isolates, Lactiplantibacillus plantarum DPU-MWM14-103 (L. plantarum M14-103) demonstrated strong acid and bile resistance, high hydrophobicity, robust autoaggregation, and potent DPPH radical scavenging activity. Genome analysis revealed the absence of antibiotic resistance and virulence genes, along with the presence of genes associated with stress response and adhesion. In vitro assays confirmed its non-toxic nature, lack of hemolytic activity, and ability to enhance tight junction integrity and reduce inflammatory cytokine expression under LPS stimulation. As a result, L. plantarum M14-103 would be a potential probiotic strain for gut health.