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<p>A novel Gram-stain-positive, strictly anaerobic, non-motile, rod-shaped bacterium, designated strain BG-O-102ᵀ, was isolated from the feces of a healthy adult. The strain showed optimal growth at 30–37&#xa0;°C and tolerated a broad pH range, with best growth occurring between pH 5.5 and 8.0. Phylogenetic analysis of the nearly full-length 16S rRNA gene (1,443&#xa0;bp) placed BG-O-102ᵀ within the genus <i>Collinsella</i>, showing highest similarity to <i>Collinsella aerofaciens</i> ATCC 25986ᵀ (97.4%). The draft genome (2,275,586&#xa0;bp) had a DNA G + C content of 60.1&#xa0;mol%, and genome-based relatedness indices (ANI &lt; 95%; dDDH &lt; 70%) clearly supported its taxonomic distinctiveness from recognized species of the genus. Strain BG-O-102ᵀ displayed marked gastrointestinal resilience, maintaining viability under simulated gastric conditions (pH 2.0–3.0; pH 2.5 for 4&#xa0;h), in 0.3% (w/v) oxgall, and in simulated intestinal fluid (pH 8.0 for 6&#xa0;h). Functional assays revealed measurable biological activities, including DPPH radical-scavenging capacity (38.7 ± 5.5%), <i>α</i>-amylase inhibition (60.5 ± 4.6%), and anti-inflammatory potential (42.9 ± 4.6% inhibition of albumin denaturation). In LPS-stimulated RAW 264.7 macrophages, BG-O-102ᵀ reduced nitric oxide production to 36.9 ± 2.3% compared with 51.0 ± 4.1% in LPS-only controls. The major cellular fatty acids were C<sub>12:0,</sub>C<sub>14:0</sub> DMA, and C<sub>16:0</sub> DMA. Based on integrated phylogenetic, genomic, chemotaxonomic, and phenotypic evidence, strain BG-O-102ᵀ represents a distinct lineage within the genus <i>Collinsella,</i> for which the name <i>Collinsella faecihominis</i> sp. nov. is proposed. The type strain is BG-O-102ᵀ (= KCTC 25436ᵀ = CGMCC 1.65152ᵀ).</p>

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Genomic and Functional Characterization of a Novel Gut-Derived Member of the Genus Collinsella With Probiotic-Associated Potential

  • 期刊论文

  • 发表日期：

    2026年3月31日

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  • Md Shamsuzzaman,
  • Ram Hari Dahal,
  • Jungmin Kim

摘要

A novel Gram-stain-positive, strictly anaerobic, non-motile, rod-shaped bacterium, designated strain BG-O-102ᵀ, was isolated from the feces of a healthy adult. The strain showed optimal growth at 30–37 °C and tolerated a broad pH range, with best growth occurring between pH 5.5 and 8.0. Phylogenetic analysis of the nearly full-length 16S rRNA gene (1,443 bp) placed BG-O-102ᵀ within the genus Collinsella, showing highest similarity to Collinsella aerofaciens ATCC 25986ᵀ (97.4%). The draft genome (2,275,586 bp) had a DNA G + C content of 60.1 mol%, and genome-based relatedness indices (ANI < 95%; dDDH < 70%) clearly supported its taxonomic distinctiveness from recognized species of the genus. Strain BG-O-102ᵀ displayed marked gastrointestinal resilience, maintaining viability under simulated gastric conditions (pH 2.0–3.0; pH 2.5 for 4 h), in 0.3% (w/v) oxgall, and in simulated intestinal fluid (pH 8.0 for 6 h). Functional assays revealed measurable biological activities, including DPPH radical-scavenging capacity (38.7 ± 5.5%), α-amylase inhibition (60.5 ± 4.6%), and anti-inflammatory potential (42.9 ± 4.6% inhibition of albumin denaturation). In LPS-stimulated RAW 264.7 macrophages, BG-O-102ᵀ reduced nitric oxide production to 36.9 ± 2.3% compared with 51.0 ± 4.1% in LPS-only controls. The major cellular fatty acids were C12:0,C14:0 DMA, and C16:0 DMA. Based on integrated phylogenetic, genomic, chemotaxonomic, and phenotypic evidence, strain BG-O-102ᵀ represents a distinct lineage within the genus Collinsella, for which the name Collinsella faecihominis sp. nov. is proposed. The type strain is BG-O-102ᵀ (= KCTC 25436ᵀ = CGMCC 1.65152ᵀ).