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<p>Rutin shows potential for reducing uric acid (UA) but suffers from low bioavailability. Microbial biotransformation can convert rutin into metabolites with enhanced absorption, stability, and physiological activity. This study aims to investigate the biotransformation effects of probiotics containing α-L-rhamnose glycosidase and α-L-rhamnose glycosidase on rutin, as well as their therapeutic efficacy for hyperuricemia (HUA). In vitro, <i>Lactobacillus gasseri</i> CCFM1346 exhibited significantly higher conversion rates for transforming rutin into quercetin and isoquercetin compared to other strains. In HUA mice, both the CCFM1346-fermented rutin preparation (post-Rutin) and a synbiotic combination (Synbiotic) significantly lowered serum UA and creatinine levels (<i>p</i> &lt; 0.05). Moreover, the Synbiotics group achieved superior serum UA reduction (74.7%) compared to the post-Rutin group (60.1%), despite more moderate xanthine oxidase inhibition. These treatments effectively decreased hepatic xanthine oxidase and enhanced urate oxidase activity, as well as downregulated mRNA levels of UA reabsorption transporters (<i>URAT1</i> and <i>GLUT9</i>) while upregulating the efflux transporter <i>ABCG2</i>. Moreover, the combination of <i>L. gasseri</i> CCFM1346 with rutin significantly inhibited the transcription of renal inflammasome components (<i>NLRP3</i>, <i>ASC</i>, <i>Caspase1</i>) and inflammatory cytokines (<i>IL-6</i>, <i>IL-1β</i>, <i>TNF-α</i>). Futuremore, correlation analysis indicates that these indicators exhibit a significant positive correlation (<i>p</i> &lt; 0.001). In conclusion, <i>L. gasseri</i> CCFM1346-fermented rutin alleviates HUA through multi-mechanistic actions, with the synbiotic preparation demonstrating superior efficacy by concurrently targeting UA production, excretion, and inflammation. These findings provide a foundation for developing synbiotic functional foods for UA management.</p>

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Effects and Mechanism of Lactobacillus gasseri CCFM1346 Combined with Rutin on Alleviating Hyperuricemia in Mice

  • 期刊论文

  • 发表日期：

    2026年4月10日

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  • Xin Tang,
  • Shuling Zhang,
  • Bingyong Mao,
  • Qiuxiang Zhang,
  • Jianxin Zhao,
  • Wei Chen,
  • Shumao Cui

摘要

Rutin shows potential for reducing uric acid (UA) but suffers from low bioavailability. Microbial biotransformation can convert rutin into metabolites with enhanced absorption, stability, and physiological activity. This study aims to investigate the biotransformation effects of probiotics containing α-L-rhamnose glycosidase and α-L-rhamnose glycosidase on rutin, as well as their therapeutic efficacy for hyperuricemia (HUA). In vitro, Lactobacillus gasseri CCFM1346 exhibited significantly higher conversion rates for transforming rutin into quercetin and isoquercetin compared to other strains. In HUA mice, both the CCFM1346-fermented rutin preparation (post-Rutin) and a synbiotic combination (Synbiotic) significantly lowered serum UA and creatinine levels (p < 0.05). Moreover, the Synbiotics group achieved superior serum UA reduction (74.7%) compared to the post-Rutin group (60.1%), despite more moderate xanthine oxidase inhibition. These treatments effectively decreased hepatic xanthine oxidase and enhanced urate oxidase activity, as well as downregulated mRNA levels of UA reabsorption transporters (URAT1 and GLUT9) while upregulating the efflux transporter ABCG2. Moreover, the combination of L. gasseri CCFM1346 with rutin significantly inhibited the transcription of renal inflammasome components (NLRP3, ASC, Caspase1) and inflammatory cytokines (IL-6, IL-1β, TNF-α). Futuremore, correlation analysis indicates that these indicators exhibit a significant positive correlation (p < 0.001). In conclusion, L. gasseri CCFM1346-fermented rutin alleviates HUA through multi-mechanistic actions, with the synbiotic preparation demonstrating superior efficacy by concurrently targeting UA production, excretion, and inflammation. These findings provide a foundation for developing synbiotic functional foods for UA management.