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<p>Probiotics possess the potential to enhance gastrointestinal health and inhibit the colonization of pathogenic microorganisms. However, the role and mechanism of <i>Lactobacillus reuteri</i> in inhibiting <i>Salmonella enteritidis</i> colonization is unknown. In this study, we isolated and characterized <i>L. reuteri</i> strain DY25 from goat feces and evaluated its probiotic properties and protective effects against <i>Salmonella</i> infection in a mouse model. The strain demonstrates elevated survival rates in simulated gastrointestinal fluids, exhibits significant hydrophobicity and self-aggregation properties, and shows robust adhesion to intestinal epithelial cells. Additionally, <i>L. reuteri</i> DY25 demonstrated significant antibacterial activity against <i>S. enteritidis</i> and other pathogenic bacteria. In vivo experiments revealed that the administration of <i>L. reuteri</i> DY25 resulted in enhanced daily weight gain, increased jejunal villus length, and an elevated villus height-to-crypt depth (V/C) ratio in mice. <i>L. reuteri</i> also modulated the gut microbiota by increasing the <i>Firmicutes</i>-to-<i>Bacteroidota</i> (F/B) ratio and enhancing the abundance of beneficial <i>Lactobacillus</i> species. Furthermore, <i>L. reuteri</i> DY25 mitigated <i>Salmonella</i>-induced intestinal inflammation by downregulating the expression of pro-inflammatory cytokines, including IL-6, IL-1β, TNF-α, and IFN-γ, and by attenuating histopathological damage in both the jejunum and colon. These findings highlight the excellent probiotic properties of <i>L. reuteri</i> DY25, underscoring its potential as a probiotic supplement for preventing and treating animal diseases such as diarrhea.</p>

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Protective Effects of Lactobacillus Reuteri Isolated from Goats in a Murine Model of Salmonella Infection

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Qibing Gu,
  • Chunjin Xiao,
  • Taichun Gao,
  • Xue Sha,
  • Kegu Ji’e,
  • Lanzhi Huang,
  • Wentao Zhang,
  • Jiao Zhang,
  • Falong Yang

摘要

Probiotics possess the potential to enhance gastrointestinal health and inhibit the colonization of pathogenic microorganisms. However, the role and mechanism of Lactobacillus reuteri in inhibiting Salmonella enteritidis colonization is unknown. In this study, we isolated and characterized L. reuteri strain DY25 from goat feces and evaluated its probiotic properties and protective effects against Salmonella infection in a mouse model. The strain demonstrates elevated survival rates in simulated gastrointestinal fluids, exhibits significant hydrophobicity and self-aggregation properties, and shows robust adhesion to intestinal epithelial cells. Additionally, L. reuteri DY25 demonstrated significant antibacterial activity against S. enteritidis and other pathogenic bacteria. In vivo experiments revealed that the administration of L. reuteri DY25 resulted in enhanced daily weight gain, increased jejunal villus length, and an elevated villus height-to-crypt depth (V/C) ratio in mice. L. reuteri also modulated the gut microbiota by increasing the Firmicutes-to-Bacteroidota (F/B) ratio and enhancing the abundance of beneficial Lactobacillus species. Furthermore, L. reuteri DY25 mitigated Salmonella-induced intestinal inflammation by downregulating the expression of pro-inflammatory cytokines, including IL-6, IL-1β, TNF-α, and IFN-γ, and by attenuating histopathological damage in both the jejunum and colon. These findings highlight the excellent probiotic properties of L. reuteri DY25, underscoring its potential as a probiotic supplement for preventing and treating animal diseases such as diarrhea.