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a <p>Immunosuppressant medications disrupt intestinal homeostasis. Probiotics may help restore intestinal balance; however, the safety of their use in immunocompromised individuals is questionable. To investigate the translocation of <i>Lacticaseibacillus rhamnosus</i> radiolabeled with technetium-99m (<sup>99m</sup>Tc) in an experimental model of immunosuppression. Mice were divided into four groups: control (CTL), 5-fluorouracil (5-FU), dexamethasone (DEX), and DEX + 5-FU. Mice in the CTL group received saline (0.1 mL/intraperitoneal [IP]) for seven days; those in the 5-FU group received a single dose of 5-fluorouracil (300 mg/kg/IP) on day 5; those in the DEX group received dexamethasone (5 mg/kg/IP) daily; and those in the DEX + 5-FU group received a single dose of 5-fluorouracil (300 mg/kg/IP) on day 5 and dexamethasone (5 mg/kg/IP) daily. On day 8, the animals received radiolabeled L. <i>rhamnosus</i> (99mTc-L. <i>rhamnosus</i>) by oral gavage. Four hours later, the mice were anesthetized and euthanized for blood and tissue collection. The 5-FU group exhibited leukopenia, whereas the DEX and DEX + 5-FU groups showed granulocytosis and lymphopenia. Radiolabeling demonstrated high stability. Significant probiotic translocation occurred in the 5-FU group, whereas no translocation was observed in the DEXgroup. Intermediate levels of translocation were detected in the DEX + 5-FU group. As expected, 5-FU treatment induced pronounced epithelialinjury, characterized by increased intestinal permeability, oxidative stress, histological damage, and inflammatory infiltration. In the DEX + 5-FUgroup, although histological and morphometric alterations were similar to those observed in the 5-FU group, and peroxidase activity wasincreased with an intermediate reduction in superoxide dismutase levels, intestinal permeability remained unchanged.</p>

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Assessment of Translocation of Lacticaseibacillus rhamnosus CGMCC 1.3724 Radiolabeled with Technetium-99 m Isotope in an Experimental Model of Immunosuppression

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月17日

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  • Nayara Salgado Vieira Sette,
  • Maria Emília Rabelo Andrade,
  • Luísa Martins Trindade,
  • Amanda Dias Borges,
  • Carla Carolina Martins Carneiro,
  • Bárbara Gatti Cardoso,
  • Simone Odília Antunes Fernandes,
  • Bruno Galloti,
  • Flaviano dos Santos Martins,
  • Paola Caroline Lacerda Leocádio,
  • Jacqueline Isaura Alvarez-Leite,
  • Geovanni Dantas Cassali,
  • Filipe Resende,
  • Vivian Vasconcelos Costa,
  • Simone de Vasconcelos Generoso,
  • Valbert Nascimento Cardoso

摘要

a

Immunosuppressant medications disrupt intestinal homeostasis. Probiotics may help restore intestinal balance; however, the safety of their use in immunocompromised individuals is questionable. To investigate the translocation of Lacticaseibacillus rhamnosus radiolabeled with technetium-99m (99mTc) in an experimental model of immunosuppression. Mice were divided into four groups: control (CTL), 5-fluorouracil (5-FU), dexamethasone (DEX), and DEX + 5-FU. Mice in the CTL group received saline (0.1 mL/intraperitoneal [IP]) for seven days; those in the 5-FU group received a single dose of 5-fluorouracil (300 mg/kg/IP) on day 5; those in the DEX group received dexamethasone (5 mg/kg/IP) daily; and those in the DEX + 5-FU group received a single dose of 5-fluorouracil (300 mg/kg/IP) on day 5 and dexamethasone (5 mg/kg/IP) daily. On day 8, the animals received radiolabeled L. rhamnosus (99mTc-L. rhamnosus) by oral gavage. Four hours later, the mice were anesthetized and euthanized for blood and tissue collection. The 5-FU group exhibited leukopenia, whereas the DEX and DEX + 5-FU groups showed granulocytosis and lymphopenia. Radiolabeling demonstrated high stability. Significant probiotic translocation occurred in the 5-FU group, whereas no translocation was observed in the DEXgroup. Intermediate levels of translocation were detected in the DEX + 5-FU group. As expected, 5-FU treatment induced pronounced epithelialinjury, characterized by increased intestinal permeability, oxidative stress, histological damage, and inflammatory infiltration. In the DEX + 5-FUgroup, although histological and morphometric alterations were similar to those observed in the 5-FU group, and peroxidase activity wasincreased with an intermediate reduction in superoxide dismutase levels, intestinal permeability remained unchanged.