Scholarlink

<p>Isolation of beneficial probiotics from traditional foods is a priority in functional food research. We isolated and characterized a lactic acid bacteria (LAB) probiotic strain from Egyptian cuisine, with therapeutic applications for alleviating the manifestations of metabolic syndrome (MetS), including hyperglycemia, hypercholesterolemia and obesity. LAB (<i>n</i> = 10) were isolated from 12 food and juice samples, identified and assessed in vitro for glucose- and cholesterol-lowering capabilities. The most promising isolate underwent probiotic characterization, including gastrointestinal tolerance, surface hydrophobicity, auto-aggregation, and milk fermentation capacity. Other beneficial properties, such as exopolysaccharide production and antimicrobial activity, were also tested. The selected isolate was evaluated for a hypoglycemic and hypocholesterolemic effect using a high-fat diet/streptozotocin-induced hypercholesterolemia and diabetes model in Wistar rats. <i>Lactiplantibacillus plantarum</i> Y<sub>10b</sub>, isolated from Egyptian cottage cheese, reduced glucose and cholesterol levels in vitro by 53 ± 0.47% and 98 ± 0.18%. It showed good probiotic characteristics: minimal viability loss in simulated gastrointestinal conditions (0.07 and 0.08 log<sub>10</sub> CFU/mL), good hydrophobicity (&gt; 70%), high auto-aggregation (82.6 ± 0.86% after 24&#xa0;h), positive exopolysaccharide production, milk fermentation capability with 21-day storage stability, and an antimicrobial activity against <i>Staphylococcus aureus</i> ATCC 25923, <i>Salmonella enterica</i> ATCC 14028, <i>Klebsiella pneumoniae</i> ATCC 10031, and <i>Escherichia coli</i> ATCC 25922. In vivo administration of <i>L. plantarum</i> Y<sub>10b</sub> in a MetS rat model resulted in significant hypoglycemic, hypocholesterolemic, and anti-obesity effects. In conclusion, <i>L. plantarum</i> Y<sub>10b</sub> is a promising probiotic for managing MetS manifestations. Further clinical investigations for use as a therapeutic intervention are highly recommended.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A Potential Probiotic Lactiplantibacillus Plantarum Isolate from Egyptian Cottage Cheese Alleviates Metabolic Syndrome Manifestations: In Vitro and In Vivo Characterization

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月24日

查看全文
  • Yehya Abdel-Moniem,
  • Kareem A. Ibrahim,
  • Omneya M. Helmy,
  • Mona T. Kashef

摘要

Isolation of beneficial probiotics from traditional foods is a priority in functional food research. We isolated and characterized a lactic acid bacteria (LAB) probiotic strain from Egyptian cuisine, with therapeutic applications for alleviating the manifestations of metabolic syndrome (MetS), including hyperglycemia, hypercholesterolemia and obesity. LAB (n = 10) were isolated from 12 food and juice samples, identified and assessed in vitro for glucose- and cholesterol-lowering capabilities. The most promising isolate underwent probiotic characterization, including gastrointestinal tolerance, surface hydrophobicity, auto-aggregation, and milk fermentation capacity. Other beneficial properties, such as exopolysaccharide production and antimicrobial activity, were also tested. The selected isolate was evaluated for a hypoglycemic and hypocholesterolemic effect using a high-fat diet/streptozotocin-induced hypercholesterolemia and diabetes model in Wistar rats. Lactiplantibacillus plantarum Y10b, isolated from Egyptian cottage cheese, reduced glucose and cholesterol levels in vitro by 53 ± 0.47% and 98 ± 0.18%. It showed good probiotic characteristics: minimal viability loss in simulated gastrointestinal conditions (0.07 and 0.08 log10 CFU/mL), good hydrophobicity (> 70%), high auto-aggregation (82.6 ± 0.86% after 24 h), positive exopolysaccharide production, milk fermentation capability with 21-day storage stability, and an antimicrobial activity against Staphylococcus aureus ATCC 25923, Salmonella enterica ATCC 14028, Klebsiella pneumoniae ATCC 10031, and Escherichia coli ATCC 25922. In vivo administration of L. plantarum Y10b in a MetS rat model resulted in significant hypoglycemic, hypocholesterolemic, and anti-obesity effects. In conclusion, L. plantarum Y10b is a promising probiotic for managing MetS manifestations. Further clinical investigations for use as a therapeutic intervention are highly recommended.