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<p>Exopolysaccharides (EPSs) have recently emerged as significant substances due to their remarkable biological properties. This research aimed to analyze EPS B151 extracted from <i>Levilactobacillus brevis</i> B151 and evaluate its biological activities. EPS B151 was obtained at a yield of 438&#xa0;mg/L. Structural analysis using the Congo red method and Fourier transform infrared spectroscopy (FTIR) revealed a random coil structure and characteristic typical carbohydrate peaks (O-H, C-H, C = O, and C-O-C), along with a pyranose ring containing both α- and β-glycosidic bonds. Scanning electron microscopy (SEM) revealed a porous, irregular, and loosely compact morphology. High-pressure liquid chromatography (HPLC) analysis indicated that EPS B151 is a heteropolysaccharide composed of mannose, ribose, glucosamine, glucuronic acid, glucose, galactose, and the rare sugar fucose. This unique composition is the first reported for an <i>L. brevis</i>-derived EPS. EPS B151 demonstrated significant antimicrobial activity against <i>Staphylococcus aureus</i> KFRI00188 and <i>Listeria monocytogenes</i> ATCC3596, inhibiting growth and cell viability in a dose-dependent manner, with bactericidal effects confirmed by propidium iodide staining and SEM analysis. It also inhibited biofilm formation (up to 58.09 ± 2.97% for <i>Staph. aureus</i> and 51.53 ± 3.84% for <i>L. monocytogenes</i>) in a dose-dependent manner. Furthermore, EPS B151 demonstrated potent antioxidant activity in multiple in vitro assays, including ABTS<sup>•+</sup>, DPPH, and OH<sup>•</sup> radical-scavenging, along with significant reducing power. In cytotoxicity assays, EPS B151 was non-toxic to normal colon cells (CCD-18Co) but showed significant inhibitory effects against colon cancer (Caco-2) and gastric cancer (AGS) cell lines, suggesting its potential anticancer properties. Overall, EPS B151 is a multifunctional bioactive compound with antimicrobial, antibiofilm, antioxidant, and anticancer properties, highlighting its potential as a functional ingredient in the food, biomedical, and pharmaceutical industries.</p>

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A Fucose-containing Hetero-exopolysaccharide From Levilactobacillus brevis B151 Exhibits Potent Antimicrobial, Antibiofilm, and Antioxidant Activities

  • 期刊论文

  • 发表日期：

    2025年11月12日

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  • Manoj Kumar Yadav,
  • Remilyn M. Mendoza,
  • Ji Hoon Song,
  • Robie Vasquez,
  • Jae Seung Lee,
  • Yeong-Seo Park,
  • Hyun-Dong Paik,
  • In Ho Kim,
  • Dae‑Kyung Kang

摘要

Exopolysaccharides (EPSs) have recently emerged as significant substances due to their remarkable biological properties. This research aimed to analyze EPS B151 extracted from Levilactobacillus brevis B151 and evaluate its biological activities. EPS B151 was obtained at a yield of 438 mg/L. Structural analysis using the Congo red method and Fourier transform infrared spectroscopy (FTIR) revealed a random coil structure and characteristic typical carbohydrate peaks (O-H, C-H, C = O, and C-O-C), along with a pyranose ring containing both α- and β-glycosidic bonds. Scanning electron microscopy (SEM) revealed a porous, irregular, and loosely compact morphology. High-pressure liquid chromatography (HPLC) analysis indicated that EPS B151 is a heteropolysaccharide composed of mannose, ribose, glucosamine, glucuronic acid, glucose, galactose, and the rare sugar fucose. This unique composition is the first reported for an L. brevis-derived EPS. EPS B151 demonstrated significant antimicrobial activity against Staphylococcus aureus KFRI00188 and Listeria monocytogenes ATCC3596, inhibiting growth and cell viability in a dose-dependent manner, with bactericidal effects confirmed by propidium iodide staining and SEM analysis. It also inhibited biofilm formation (up to 58.09 ± 2.97% for Staph. aureus and 51.53 ± 3.84% for L. monocytogenes) in a dose-dependent manner. Furthermore, EPS B151 demonstrated potent antioxidant activity in multiple in vitro assays, including ABTS•+, DPPH, and OH• radical-scavenging, along with significant reducing power. In cytotoxicity assays, EPS B151 was non-toxic to normal colon cells (CCD-18Co) but showed significant inhibitory effects against colon cancer (Caco-2) and gastric cancer (AGS) cell lines, suggesting its potential anticancer properties. Overall, EPS B151 is a multifunctional bioactive compound with antimicrobial, antibiofilm, antioxidant, and anticancer properties, highlighting its potential as a functional ingredient in the food, biomedical, and pharmaceutical industries.