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<p>Enterotoxigenic <i>Escherichia coli</i> K88 (ETEC K88), a member of the <i>E. coli</i> family, poses a serious threat to human health and animal husbandry due to its high morbidity and mortality rates. Herein, three strains of lactic acid bacteria were isolated from the feces of Luhua chickens raised on farms in the Inner Mongolia Autonomous Region, China. Their antibacterial spectrum, acid resistance, bile salt resistance, antibiotic sensitivity, and other biological characteristics were evaluated. The results showed that <i>Ligilactobacillus salivarius</i> X9 (<i>L. salivarius</i> X9) exhibited superior acid and bile salt tolerance, favorable safety properties, and the strongest antibacterial activity against multidrug-resistance ETEC K88. Therefore, this study evaluated whether <i>L. salivarius</i> X9 could mitigate intestinal inflammatory damage induced by ETEC K88 in mice. The results showed that IL-1β levels were significantly reduced in the <i>L. salivarius</i> X9 treatment group compared with the intragastric ETEC K88 model group (GI group), while IL-10 levels were significantly upregulated in the treatment group. In addition, preconditioning with <i>L. salivarius</i> X9 increased the relative abundance of gut probiotics, regulated the accumulation of L-arginine and stearic acid, alleviated morphological damage in the mouse ileum induced by ETEC K88, and prevented intestinal inflammation. Our findings provide a foundation for the clinical application of <i>L. salivarius</i> X9 in the prevention of intestinal inflammation caused by ETEC K88.</p>

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Non-targeted Metabolomic and Intestinal Microbiota Analysis of Ligilactobacillus salivarius X9 in Enterotoxigenic Escherichia coli K88-induced Enteritis in Mice

  • 期刊论文

  • 发表日期：

    2025年11月19日

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  • Weixue Meng,
  • Nan Dai,
  • Leiyilan Wen,
  • Tajin Wang,
  • Lei Qu,
  • Lili Guan,
  • Jingrui Chen,
  • Lingcong Kong,
  • Hongxia Ma,
  • Haipeng Zhang

摘要

Enterotoxigenic Escherichia coli K88 (ETEC K88), a member of the E. coli family, poses a serious threat to human health and animal husbandry due to its high morbidity and mortality rates. Herein, three strains of lactic acid bacteria were isolated from the feces of Luhua chickens raised on farms in the Inner Mongolia Autonomous Region, China. Their antibacterial spectrum, acid resistance, bile salt resistance, antibiotic sensitivity, and other biological characteristics were evaluated. The results showed that Ligilactobacillus salivarius X9 (L. salivarius X9) exhibited superior acid and bile salt tolerance, favorable safety properties, and the strongest antibacterial activity against multidrug-resistance ETEC K88. Therefore, this study evaluated whether L. salivarius X9 could mitigate intestinal inflammatory damage induced by ETEC K88 in mice. The results showed that IL-1β levels were significantly reduced in the L. salivarius X9 treatment group compared with the intragastric ETEC K88 model group (GI group), while IL-10 levels were significantly upregulated in the treatment group. In addition, preconditioning with L. salivarius X9 increased the relative abundance of gut probiotics, regulated the accumulation of L-arginine and stearic acid, alleviated morphological damage in the mouse ileum induced by ETEC K88, and prevented intestinal inflammation. Our findings provide a foundation for the clinical application of L. salivarius X9 in the prevention of intestinal inflammation caused by ETEC K88.