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<p>A synbiotic composed of pomegranate peels extract, <i>Saccharomyces</i> (<i>S.</i>) <i>cerevisiae</i>, <i>Lactococcus</i> (<i>L.</i>) <i>lactis</i>, <i>Lactobacillus</i> (<i>Lact</i>) <i>paracasei</i>, and <i>Lact. plantarum</i> was fabricated in free (FPLYS) and (NPLYS) nano-encapsulated forms. The encapsulation efficiency, physichochemical properties, and antioxidant and antimicrobial activities of FPLYS and NPLYS were tested. For <i>in vitro</i> rumen fermentation study, a basal diet was supplemented with FPLYS-H (10<sup>9</sup> CFU probiotics/g diet), NPLYS-H (10<sup>9</sup> CFU probiotics/g diet), NPLYS-L (10<sup>7</sup> CFU probiotics/g diet), 40&#xa0;mg/kg diet monensin (MONS), or not supplemented (CON). The encapsulation efficiency of gum arabic-gelatin complex for prebiotic and probiotics was 75.26% and 91.80%, respectively. The size and zeta potential of gum arabic-gelatin were 27.72&#xa0;nm and 11 mV, respectively. NPLYS showed two-time higher antioxidant activity than FPLYS. NPLYS showed antimicrobial activity against <i>Escherichia</i> (<i>E.</i>) <i>coli</i> and <i>Staphylococcus</i> (<i>Staph.</i>) <i>aureus</i> similar to gentamicin, while FPLYS showed lower activity (<i>p &lt;</i> 0.05). NPLYS and FPLYS showed antimicrobial activity against <i>Pseudomonas</i> (<i>Ps.</i>) <i>aeruginosa</i> and antifungal activity against <i>Aspergillus</i> (<i>A.</i>) <i>niger</i> and <i>A. flavus</i>, but lower than getntamicin and fluconazol, respectively (<i>p &lt;</i> 0.05). NPLYS-L significantly increased total gas production compared to CON, MONS, and NPLYS-H. Compared to CON, both MONS and FPLYS-H significantly reduced methane production, followed by NPLYS-H and NPLYS-L. Among treatments, MONS significantly increased propionate, while reducing butyrate and acetate/propionate ration. All treatments increased cellulose digestibility and protozoal count (<i>p</i> &lt; 0.05). NPLYS-L significantly decreased rumen ammonia concentration compared to CON, however other treatments recorded intermediate values. The synbiotic developed in this study can be either used as an antimicrobial agent and/or a rumen fermentation modifier, replacing synthetic antibiotics used for these purposes. Nanoencapsulation process improved biological activities of the synbiotic.</p>

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Assessment of a Novel Synbitotic of Pomegranate Peels Extract and Multi-Specie Probiotics as an Antibiotics Alternative in Ruminants

  • 期刊论文

  • 发表日期：

    2025年12月11日

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  • Nesrein M. Hashem,
  • Nourhan S. Hosny,
  • Nagwa El-Desoky,
  • Gomaa Abo El Lail,
  • Yosra A. Soltan,
  • Ahmed A. Elolimy,
  • El-Sayed M. Abu-Tor,
  • Sobhy M. A. Sallam

摘要

A synbiotic composed of pomegranate peels extract, Saccharomyces (S.) cerevisiae, Lactococcus (L.) lactis, Lactobacillus (Lact) paracasei, and Lact. plantarum was fabricated in free (FPLYS) and (NPLYS) nano-encapsulated forms. The encapsulation efficiency, physichochemical properties, and antioxidant and antimicrobial activities of FPLYS and NPLYS were tested. For in vitro rumen fermentation study, a basal diet was supplemented with FPLYS-H (109 CFU probiotics/g diet), NPLYS-H (109 CFU probiotics/g diet), NPLYS-L (107 CFU probiotics/g diet), 40 mg/kg diet monensin (MONS), or not supplemented (CON). The encapsulation efficiency of gum arabic-gelatin complex for prebiotic and probiotics was 75.26% and 91.80%, respectively. The size and zeta potential of gum arabic-gelatin were 27.72 nm and 11 mV, respectively. NPLYS showed two-time higher antioxidant activity than FPLYS. NPLYS showed antimicrobial activity against Escherichia (E.) coli and Staphylococcus (Staph.) aureus similar to gentamicin, while FPLYS showed lower activity (p < 0.05). NPLYS and FPLYS showed antimicrobial activity against Pseudomonas (Ps.) aeruginosa and antifungal activity against Aspergillus (A.) niger and A. flavus, but lower than getntamicin and fluconazol, respectively (p < 0.05). NPLYS-L significantly increased total gas production compared to CON, MONS, and NPLYS-H. Compared to CON, both MONS and FPLYS-H significantly reduced methane production, followed by NPLYS-H and NPLYS-L. Among treatments, MONS significantly increased propionate, while reducing butyrate and acetate/propionate ration. All treatments increased cellulose digestibility and protozoal count (p < 0.05). NPLYS-L significantly decreased rumen ammonia concentration compared to CON, however other treatments recorded intermediate values. The synbiotic developed in this study can be either used as an antimicrobial agent and/or a rumen fermentation modifier, replacing synthetic antibiotics used for these purposes. Nanoencapsulation process improved biological activities of the synbiotic.